10.3389/fimmu.2019.01890.s003
David DeWolfe
David
DeWolfe
Malika Aid
Malika
Aid
Kevin McGann
Kevin
McGann
Joshua Ghofrani
Joshua
Ghofrani
Emma Geiger
Emma
Geiger
Catherine Helzer
Catherine
Helzer
Shaily Malik
Shaily
Malik
Steve Kleiboeker
Steve
Kleiboeker
Stephanie Jost
Stephanie
Jost
Chen Sabrina Tan
Chen Sabrina
Tan
Data_Sheet_3_NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates.docx
Frontiers
2019
NK cells
immune risk profile
BK virus
kidney transplant
immune senescence
end stage renal disease
dialysis
2019-08-23 11:46:10
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_3_NK_Cells_Contribute_to_the_Immune_Risk_Profile_in_Kidney_Transplant_Candidates_docx/9724865
<p>Background: A previously proposed immune risk profile (IRP), based on T cell phenotype and CMV serotype, is associated with mortality in the elderly and increased infections post-kidney transplant. To evaluate if NK cells contribute to the IRP and if the IRP can be predicted by a clinical T cell functional assays, we conducted a cross sectional study in renal transplant candidates to determine the incidence of IRP and its association with specific NK cell characteristics and ImmuKnow® value.</p><p>Material and Methods: Sixty five subjects were enrolled in 5 cohorts designated by age and dialysis status. We determined T and NK cell phenotypes by flow cytometry and analyzed multiple factors contributing to IRP.</p><p>Results: We identified 14 IRP+ [CMV seropositivity and CD4/CD8 ratio < 1 or being in the highest quintile of CD8+ senescent (28CD–/CD57+) T cells] individuals equally divided amongst the cohorts. Multivariable linear regression revealed a distinct IRP+ group. Age and dialysis status did not predict immune senescence in kidney transplant candidates. NK cell features alone could discriminate IRP– and IRP+ patients, suggesting that NK cells significantly contribute to the overall immune status in kidney transplant candidates and that a combined T and NK cell phenotyping can provide a more detailed IRP definition. ImmuKnow® value was negatively correlated to age and significantly lower in IRP+ patients and predicts IRP when used alone or in combination with NK cell features.</p><p>Conclusion: NK cells contribute to overall immune senescence in kidney transplant candidates.</p>