10.3389/fnmol.2019.00195.s001
Eduardo Penna
Eduardo
Penna
Angela Cerciello
Angela
Cerciello
Angela Chambery
Angela
Chambery
Rosita Russo
Rosita
Russo
Filippo M. Cernilogar
Filippo M.
Cernilogar
Emilia Maria Pedone
Emilia Maria
Pedone
Carla Perrone-Capano
Carla
Perrone-Capano
Silvia Cappello
Silvia
Cappello
Rossella Di Giaimo
Rossella
Di Giaimo
Marianna Crispino
Marianna
Crispino
Table_1_Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids.xlsx
Frontiers
2019
CSTB
synaptosomes
EPM1A
cerebral organoids
synaptic plasticity
local protein synthesis
2019-08-16 14:58:01
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_Cystatin_B_Involvement_in_Synapse_Physiology_of_Rodent_Brains_and_Human_Cerebral_Organoids_xlsx/9638990
<p>Cystatin B (CSTB) is a ubiquitous protein belonging to a superfamily of protease inhibitors. CSTB may play a critical role in brain physiology because its mutations cause progressive myoclonic epilepsy-1A (EPM1A), the most common form of progressive myoclonic epilepsy. However, the molecular mechanisms underlying the role of CSTB in the central nervous system (CNS) are largely unknown. To investigate the possible involvement of CSTB in the synaptic plasticity, we analyzed its expression in synaptosomes as a model system in studying the physiology of the synaptic regions of the CNS. We found that CSTB is not only present in the synaptosomes isolated from rat and mouse brain cortex, but also secreted into the medium in a depolarization-controlled manner. In addition, using biorthogonal noncanonical amino acid tagging (BONCAT) procedure, we demonstrated, for the first time, that CSTB is locally synthesized in the synaptosomes. The synaptic localization of CSTB was confirmed in a human 3D model of cortical development, namely cerebral organoids. Altogether, these results suggest that CSTB may play a role in the brain plasticity and open a new perspective in studying the involvement of CSTB deregulation in neurodegenerative and neuropsychiatric diseases.</p>