10.3389/fnmol.2019.00195.s001 Eduardo Penna Eduardo Penna Angela Cerciello Angela Cerciello Angela Chambery Angela Chambery Rosita Russo Rosita Russo Filippo M. Cernilogar Filippo M. Cernilogar Emilia Maria Pedone Emilia Maria Pedone Carla Perrone-Capano Carla Perrone-Capano Silvia Cappello Silvia Cappello Rossella Di Giaimo Rossella Di Giaimo Marianna Crispino Marianna Crispino Table_1_Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids.xlsx Frontiers 2019 CSTB synaptosomes EPM1A cerebral organoids synaptic plasticity local protein synthesis 2019-08-16 14:58:01 Dataset https://frontiersin.figshare.com/articles/dataset/Table_1_Cystatin_B_Involvement_in_Synapse_Physiology_of_Rodent_Brains_and_Human_Cerebral_Organoids_xlsx/9638990 <p>Cystatin B (CSTB) is a ubiquitous protein belonging to a superfamily of protease inhibitors. CSTB may play a critical role in brain physiology because its mutations cause progressive myoclonic epilepsy-1A (EPM1A), the most common form of progressive myoclonic epilepsy. However, the molecular mechanisms underlying the role of CSTB in the central nervous system (CNS) are largely unknown. To investigate the possible involvement of CSTB in the synaptic plasticity, we analyzed its expression in synaptosomes as a model system in studying the physiology of the synaptic regions of the CNS. We found that CSTB is not only present in the synaptosomes isolated from rat and mouse brain cortex, but also secreted into the medium in a depolarization-controlled manner. In addition, using biorthogonal noncanonical amino acid tagging (BONCAT) procedure, we demonstrated, for the first time, that CSTB is locally synthesized in the synaptosomes. The synaptic localization of CSTB was confirmed in a human 3D model of cortical development, namely cerebral organoids. Altogether, these results suggest that CSTB may play a role in the brain plasticity and open a new perspective in studying the involvement of CSTB deregulation in neurodegenerative and neuropsychiatric diseases.</p>