10.3389/fendo.2019.00545.s001 Qing Li Qing Li Haishen Wen Haishen Wen Yun Li Yun Li Zhanxiong Zhang Zhanxiong Zhang Yangyang Zhou Yangyang Zhou Xin Qi Xin Qi Image_1_Evidence for the Direct Effect of the NPFF Peptide on the Expression of Feeding-Related Factors in Spotted Sea Bass (Lateolabrax maculatus).pdf Frontiers 2019 spotted sea bass NPFF/NPFFR system feeding in situ hybridization static incubation 2019-08-06 04:36:58 Figure https://frontiersin.figshare.com/articles/figure/Image_1_Evidence_for_the_Direct_Effect_of_the_NPFF_Peptide_on_the_Expression_of_Feeding-Related_Factors_in_Spotted_Sea_Bass_Lateolabrax_maculatus_pdf/9253652 <p>Neuropeptide FF (NPFF) is a family member of RF-amide peptides, which are suggested to be involved in the control of vertebrate feeding behavior. However, little is known about the effect of the NPFF peptide on feeding-related processes in basal vertebrates. In this study, four full-length cDNAs, npff, npffr1, npffr2-1, and npffr2-2, were cloned from spotted sea bass and characterized. The conserved NPFF peptide is biologically active because it functionally interacts with different receptors expressed in cultured eukaryotic cells to enhance CRE promoter activity. Tissue distribution analysis showed that the highest npff mRNA expression occurred in the telencephalon, hypothalamus, medulla, gonad and muscle, but the npffrs mRNAs were mainly distributed within the central nervous system (CNS). In situ hybridization (ISH) detected npff-expressing cells in several specific regions ranging across the telencephalon and midbrain to the hypothalamus. Incubation of the spotted sea bass conserved NPFF peptide significantly increased the expression of orexin (orx) and neuropeptide Y (npy) mRNA and decreased the expression of leptin (lep), somatostatin (ss), and cholecystokinin (cck) mRNA in brain cells. Similarly, the conserved NPFF peptide also heightened the expression of gastrin (gas), ghrelin (ghrl), and motilin (mtl) mRNA and significantly reduced the expression of cck mRNA in the intestine and stomach. Taken together, these data suggest that the NPFF peptide may play a stimulating role in regulating feeding-related processes in spotted sea bass.</p>