10.3389/fgene.2019.00651.s008
Priscila S.N. de Oliveira
Priscila S.N.
de Oliveira
Luiz L. Coutinho
Luiz L.
Coutinho
Aline S.M. Cesar
Aline S.M.
Cesar
Wellison J. da Silva Diniz
Wellison J.
da Silva Diniz
Marcela M. de Souza
Marcela M.
de Souza
Bruno G. Andrade
Bruno G.
Andrade
James E. Koltes
James E.
Koltes
Gerson B. Mourão
Gerson B.
Mourão
Adhemar Zerlotini
Adhemar
Zerlotini
James M. Reecy
James M.
Reecy
Luciana C.A. Regitano
Luciana C.A.
Regitano
Table_5_Co-Expression Networks Reveal Potential Regulatory Roles of miRNAs in Fatty Acid Composition of Nelore Cattle.xlsx
Frontiers
2019
Bos indicus
conjugated linoleic acid
integrative genomics
mRNA
miRNA
oleic acid
2019-07-11 13:39:31
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_5_Co-Expression_Networks_Reveal_Potential_Regulatory_Roles_of_miRNAs_in_Fatty_Acid_Composition_of_Nelore_Cattle_xlsx/8858870
<p>Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were obtained from skeletal muscle of Nelore cattle that had extreme genomic breeding values for OA and CLA. Insulin and MAPK signaling pathways were identified by WGCNA as central pathways associated with both of these fatty acids. Co-expression network analysis identified bta-miR-33a/b, bta-miR-100, bta-miR-204, bta-miR-365-5p, bta-miR-660, bta-miR-411a, bta-miR-136, bta-miR-30-5p, bta-miR-146b, bta-let-7a-5p, bta-let-7f, bta-let-7, bta-miR 339, bta-miR-10b, bta-miR 486, and the genes ACTA1 and ALDOA as potential regulators of fatty acid synthesis. This study provides evidence and insights into the molecular mechanisms and potential target genes involved in fatty acid content differences in Nelore beef cattle, revealing new candidate pathways of phenotype modulation that could positively benefit beef production and human consumption.</p>