10.3389/fmicb.2019.01427.s015
Scott C. Thomas
Scott C.
Thomas
Kevin O. Tamadonfar
Kevin O.
Tamadonfar
Cale O. Seymour
Cale O.
Seymour
Dengxun Lai
Dengxun
Lai
Jeremy A. Dodsworth
Jeremy A.
Dodsworth
Senthil K. Murugapiran
Senthil K.
Murugapiran
Emiley A. Eloe-Fadrosh
Emiley A.
Eloe-Fadrosh
Paul Dijkstra
Paul
Dijkstra
Brian P. Hedlund
Brian P.
Hedlund
Table_9_Position-Specific Metabolic Probing and Metagenomics of Microbial Communities Reveal Conserved Central Carbon Metabolic Network Activities at High Temperatures.XLSX
Frontiers
2019
13C stable isotope
isotopomers
heterotrophy
catabolic
anabolic
thermophile
hyperthermophile
diversity
2019-07-05 10:41:06
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_9_Position-Specific_Metabolic_Probing_and_Metagenomics_of_Microbial_Communities_Reveal_Conserved_Central_Carbon_Metabolic_Network_Activities_at_High_Temperatures_XLSX/8791304
<p>Temperature is a primary driver of microbial community composition and taxonomic diversity; however, it is unclear to what extent temperature affects characteristics of central carbon metabolic pathways (CCMPs) at the community level. In this study, 16S rRNA gene amplicon and metagenome sequencing were combined with <sup>13</sup>C-labeled metabolite probing of the CCMPs to assess community carbon metabolism along a temperature gradient (60–95°C) in Great Boiling Spring, NV. 16S rRNA gene amplicon diversity was inversely proportional to temperature, and Archaea were dominant at higher temperatures. KO richness and diversity were also inversely proportional to temperature, yet CCMP genes were similarly represented across the temperature gradient and many individual metagenome-assembled genomes had complete pathways. In contrast, genes encoding cellulosomes and many genes involved in plant matter degradation and photosynthesis were absent at higher temperatures. In situ<sup>13</sup>C-CO<sub>2</sub> production from labeled isotopomer pairs of glucose, pyruvate, and acetate suggested lower relative oxidative pentose phosphate pathway activity and/or fermentation at 60°C, and a stable or decreased maintenance energy demand at higher temperatures. Catabolism of <sup>13</sup>C-labeled citrate, succinate, L-alanine, L-serine, and L-cysteine was observed at 85°C, demonstrating broad heterotrophic activity and confirming functioning of the TCA cycle. Together, these results suggest that temperature-driven losses in biodiversity and gene content in geothermal systems may not alter CCMP function or maintenance energy demands at a community level.</p>