%0 Generic %A Yu, Yue %A Wang, Jia %A Kaul, Sunil C. %A Wadhwa, Renu %A Miyako, Eijiro %D 2019 %T Data_Sheet_1_Folic Acid Receptor-Mediated Targeting Enhances the Cytotoxicity, Efficacy, and Selectivity of Withania somnifera Leaf Extract: In vitro and in vivo Evidence.docx %U https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Folic_Acid_Receptor-Mediated_Targeting_Enhances_the_Cytotoxicity_Efficacy_and_Selectivity_of_Withania_somnifera_Leaf_Extract_In_vitro_and_in_vivo_Evidence_docx/8671487 %R 10.3389/fonc.2019.00602.s001 %2 https://frontiersin.figshare.com/ndownloader/files/15967706 %K Withania somnifera %K folic acid %K i-Extract %K nanomedicine %K chemotherapy %X

Nanomedicine holds great potential for drug delivery to achieve more effective and safer cancer treatment. Earlier, we reported that the alcoholic extract of Withania somnifera leaves (i-Extract) has selective cancer cell killing activity. Herein, we developed a folate receptor-targeting i-Extract nanocomplex (FRi-ExNC) that suspends well in water and possesses enhanced selective anticancer activity in both in vitro and in vivo assays. Comparative analyses of folate receptor (FR)-positive and -negative cells revealed that FRi-ExNC caused a stronger decrease in Cyclin D/Cdk4 and anti-apoptotic protein Bcl-2, as well as a higher increase in the growth arrest regulating protein p21WAF1 and pro-apoptotic protein PARP-1, in FR-enriched cancer cells. Our results demonstrate that FRi-ExNC could be a natural source-based nanomedicine for targeted cancer therapy.

%I Frontiers