10.3389/fmicb.2019.01398.s006 Zhengchao Xia Zhengchao Xia Xuelin Zhou Xuelin Zhou Jingyi Li Jingyi Li Lei Li Lei Li Yi Ma Yi Ma Yi Wu Yi Wu Zhong Huang Zhong Huang Xiaorong Li Xiaorong Li Pingxiang Xu Pingxiang Xu Ming Xue Ming Xue Table_2_Multiple-Omics Techniques Reveal the Role of Glycerophospholipid Metabolic Pathway in the Response of Saccharomyces cerevisiae Against Hypoxic Stress.DOC Frontiers 2019 hypoxia transcriptomics proteomics metabolomics Saccharomyces cerevisiae glycerophospholipid metabolism 2019-06-27 08:55:56 Dataset https://frontiersin.figshare.com/articles/dataset/Table_2_Multiple-Omics_Techniques_Reveal_the_Role_of_Glycerophospholipid_Metabolic_Pathway_in_the_Response_of_Saccharomyces_cerevisiae_Against_Hypoxic_Stress_DOC/8332403 <p>Although the biological processes of organism under hypoxic stress had been elucidated, the whole physiological changes of Saccharomyces cerevisiae are still unclear. In this work, we investigated the changes of biological process of S. cerevisiae under hypoxia by the methods of transcriptomics, proteomics, metabolomics, and bioinformatics. The results showed that the expression of a total of 1017 mRNA in transcriptome, 213 proteins in proteome, and 51 metabolites in metabolome had been significantly changed between the hypoxia and normoxia conditions. Moreover, based on the integration of system-omics data, we found that the carbohydrate, amino acids, fatty acid biosynthesis, lipid metabolic pathway, and oxidative phosphorylation were significantly changed in hypoxic stress. Among these pathways, the glycerophospholipid metabolic pathway was remarkably up-regulated from the mRNA, protein, and metabolites levels under hypoxic stress, and the expression of relevant mRNA was also confirmed by the qPCR. The metabolites of glycerophospholipid pathway such as phosphatidylcholine, phosphatidylethanolamine, phosphoinositide, and phosphatidic acids probably maintained the stability of cell membranes against hypoxic stress to relieve the cell injury, and kept S. cerevisiae survive with energy production. These findings in the hypoxic omics and integrated networks provide very useful information for further exploring the molecular mechanism of hypoxic stress.</p>