10.3389/fmicb.2019.01398.s006
Zhengchao Xia
Zhengchao
Xia
Xuelin Zhou
Xuelin
Zhou
Jingyi Li
Jingyi
Li
Lei Li
Lei
Li
Yi Ma
Yi
Ma
Yi Wu
Yi
Wu
Zhong Huang
Zhong
Huang
Xiaorong Li
Xiaorong
Li
Pingxiang Xu
Pingxiang
Xu
Ming Xue
Ming
Xue
Table_2_Multiple-Omics Techniques Reveal the Role of Glycerophospholipid Metabolic Pathway in the Response of Saccharomyces cerevisiae Against Hypoxic Stress.DOC
Frontiers
2019
hypoxia
transcriptomics
proteomics
metabolomics
Saccharomyces cerevisiae
glycerophospholipid metabolism
2019-06-27 08:55:56
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_2_Multiple-Omics_Techniques_Reveal_the_Role_of_Glycerophospholipid_Metabolic_Pathway_in_the_Response_of_Saccharomyces_cerevisiae_Against_Hypoxic_Stress_DOC/8332403
<p>Although the biological processes of organism under hypoxic stress had been elucidated, the whole physiological changes of Saccharomyces cerevisiae are still unclear. In this work, we investigated the changes of biological process of S. cerevisiae under hypoxia by the methods of transcriptomics, proteomics, metabolomics, and bioinformatics. The results showed that the expression of a total of 1017 mRNA in transcriptome, 213 proteins in proteome, and 51 metabolites in metabolome had been significantly changed between the hypoxia and normoxia conditions. Moreover, based on the integration of system-omics data, we found that the carbohydrate, amino acids, fatty acid biosynthesis, lipid metabolic pathway, and oxidative phosphorylation were significantly changed in hypoxic stress. Among these pathways, the glycerophospholipid metabolic pathway was remarkably up-regulated from the mRNA, protein, and metabolites levels under hypoxic stress, and the expression of relevant mRNA was also confirmed by the qPCR. The metabolites of glycerophospholipid pathway such as phosphatidylcholine, phosphatidylethanolamine, phosphoinositide, and phosphatidic acids probably maintained the stability of cell membranes against hypoxic stress to relieve the cell injury, and kept S. cerevisiae survive with energy production. These findings in the hypoxic omics and integrated networks provide very useful information for further exploring the molecular mechanism of hypoxic stress.</p>