10.3389/fmicb.2019.01335.s009
Zhong-Ling Wen
Zhong-Ling
Wen
Min-Kai Yang
Min-Kai
Yang
Mei-Hang Du
Mei-Hang
Du
Zhao-Zhao Zhong
Zhao-Zhao
Zhong
Yun-Ting Lu
Yun-Ting
Lu
Gu-Hao Wang
Gu-Hao
Wang
Xiao-Mei Hua
Xiao-Mei
Hua
Aliya Fazal
Aliya
Fazal
Chun-Hua Mu
Chun-Hua
Mu
Shu-Feng Yan
Shu-Feng
Yan
Yan Zhen
Yan
Zhen
Rong-Wu Yang
Rong-Wu
Yang
Jin-Liang Qi
Jin-Liang
Qi
Zhi Hong
Zhi
Hong
Gui-Hua Lu
Gui-Hua
Lu
Yong-Hua Yang
Yong-Hua
Yang
Image_6_Enrichments/Derichments of Root-Associated Bacteria Related to Plant Growth and Nutrition Caused by the Growth of an EPSPS-Transgenic Maize Line in the Field.TIF
Frontiers
2019
enrichments
derichments
root-associated bacteria
EPSPS-transgenic maize
nitrogen/phosphorus cycling
2019-06-18 04:37:53
Figure
https://frontiersin.figshare.com/articles/figure/Image_6_Enrichments_Derichments_of_Root-Associated_Bacteria_Related_to_Plant_Growth_and_Nutrition_Caused_by_the_Growth_of_an_EPSPS-Transgenic_Maize_Line_in_the_Field_TIF/8286533
<p>During the past decades, the effects of the transgenic crops on soil microbial communities have aroused widespread interest of scientists, which was mainly related to the health and growth of plants. In this study, the maize root-associated bacterial communities of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) transgenic glyphosate-tolerant (GT) maize line CC-2 (CC2) and its recipient variety Zhengdan958 (Z958) were compared at the tasseling and flowering stages by high-throughput sequencing of V3-V4 hypervariable regions of 16S rRNA gene (16S rDNA) amplicons via Illumina MiSeq. In addition, real-time quantitative PCR (qPCR) was also performed to analyze the nifH gene abundance between CC2 and Z958. Our results showed no significant difference in alpha/beta diversity of root-associated bacterial communities at the tasseling or flowering stage between CC2 and Z958 under field growth conditions. The relative abundances of the genera Bradyrhizobium and Bacillus including species B. cereus and B. muralis were significantly lower in the roots of CC2 than that of Z985 under field conditions. Both these species are regarded as plant growth promoting bacteria (PGPB), as they belong to both nitrogen-fixing and phosphate-solubilizing bacterial genera. The comparison of the relative abundance of nitrogen-fixing/phosphate-solubilizing bacteria at the class, order or family levels indicated that only one class Bacilli, one order Bacillales and one family Bacillaceae were found to be significantly lower in the roots of CC2 than that of Z985. These bacteria were also enriched in the roots and rhizospheric soil than in the surrounding soil at both two stages. Furthermore, the class Betaproteobacteria, the order Burkholderiales, the family Comamonadaceae, and the genus Acidovorax were significantly higher in the roots of CC2 than that of Z985 at the tasseling stage, meanwhile the order Burkholderiales and the family Comamonadaceae were also enriched in the roots than in the rhizospheric soil at both stages. Additionally, the nifH gene abundance at the tasseling stage in the rhizosphere soil also showed significant difference. The relative abundance of nifH gene was higher in the root samples and lower in the surrounding soil, which implicated that the roots of maize tend to be enriched in nitrogen-fixing bacteria.</p>