10.3389/fimmu.2019.01367.s006
Eliana Goncalves-Alves
Eliana
Goncalves-Alves
Victoria Saferding
Victoria
Saferding
Christopher Schliehe
Christopher
Schliehe
Robert Benson
Robert
Benson
Mariola Kurowska-Stolarska
Mariola
Kurowska-Stolarska
Julia Stefanie Brunner
Julia Stefanie
Brunner
Antonia Puchner
Antonia
Puchner
Bruno K. Podesser
Bruno K.
Podesser
Josef S. Smolen
Josef S.
Smolen
Kurt Redlich
Kurt
Redlich
Michael Bonelli
Michael
Bonelli
James Brewer
James
Brewer
Andreas Bergthaler
Andreas
Bergthaler
Günter Steiner
Günter
Steiner
Stephan Blüml
Stephan
Blüml
Video_2_MicroRNA-155 Controls T Helper Cell Activation During Viral Infection.mp4
Frontiers
2019
microRNA-155
antiviral immunity
T helper cells
T cell activation
APCs
APC-T cell interaction
2019-06-13 13:26:18
Media
https://frontiersin.figshare.com/articles/media/Video_2_MicroRNA-155_Controls_T_Helper_Cell_Activation_During_Viral_Infection_mp4/8269337
<p>MicroRNA (miR) 155 has been implicated in the regulation of innate and adaptive immunity as well as autoimmune processes. Importantly, it has been shown to regulate several antiviral responses, but its contribution to the immune response against cytopathic viruses such as vesicular stomatitis virus (VSV) infections is not known. Using transgenic/recombinant VSV expressing ovalbumin, we show that miR-155 is crucially involved in regulating the T helper cell response against this virus. Our experiments indicate that miR-155 in CD4<sup>+</sup> T cells controls their activation, proliferation, and cytokine production in vitro and in vivo upon immunization with OVA as well as during VSV viral infection. Using intravital multiphoton microscopy we analyzed the interaction of antigen presenting cells (APCs) and T cells after OVA immunization and found impaired complex formation when using miR-155 deficient CD4<sup>+</sup> T cells compared to wildtype CD4<sup>+</sup> T cells ex vivo. In contrast, miR-155 was dispensable for the maturation of myeloid APCs and for their T cell stimulatory capacity. Our data provide the first evidence that miR-155 is required for efficient CD4<sup>+</sup> T cell activation during anti-viral defense by allowing robust APC-T cell interaction required for activation and cytokine production of virus specific T cells.</p>