10.3389/fgene.2019.00563.s004
Cristine Dieter
Cristine
Dieter
Taís Silveira Assmann
Taís Silveira
Assmann
Aline Rodrigues Costa
Aline Rodrigues
Costa
Luís Henrique Canani
Luís Henrique
Canani
Bianca Marmontel de Souza
Bianca Marmontel
de Souza
Andrea Carla Bauer
Andrea Carla
Bauer
Daisy Crispim
Daisy
Crispim
Table_2_MiR-30e-5p and MiR-15a-5p Expressions in Plasma and Urine of Type 1 Diabetic Patients With Diabetic Kidney Disease.XLS
Frontiers
2019
microRNA expression
miR-15a-5p
miR-30e-5p
diabetic kidney disease
bioinformatics analysis
type 1 diabetes mellitus
2019-06-12 09:57:34
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_2_MiR-30e-5p_and_MiR-15a-5p_Expressions_in_Plasma_and_Urine_of_Type_1_Diabetic_Patients_With_Diabetic_Kidney_Disease_XLS/8262479
Introduction<p>Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR-15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR-15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation.</p>Methods<p>MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software.</p>Results<p>MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia.</p>Conclusion<p>MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD.</p>