10.3389/fcimb.2019.00178.s001
Si Liu
Si
Liu
Chaozeng Si
Chaozeng
Si
Yang Yu
Yang
Yu
Guiping Zhao
Guiping
Zhao
Lei Chen
Lei
Chen
Yu Zhao
Yu
Zhao
Zheng Zhang
Zheng
Zhang
Hengcun Li
Hengcun
Li
Yang Chen
Yang
Chen
Li Min
Li
Min
Shutian Zhang
Shutian
Zhang
Shengtao Zhu
Shengtao
Zhu
Image_1_Multi-omics Analysis of Gut Microbiota and Metabolites in Rats With Irritable Bowel Syndrome.TIF
Frontiers
2019
irritable bowel syndrome (IBS)
water avoidance stress (WAS)
fecal metabolomics profiling
16S rRNA gene sequencing
correlation analysis
2019-05-29 10:43:47
Figure
https://frontiersin.figshare.com/articles/figure/Image_1_Multi-omics_Analysis_of_Gut_Microbiota_and_Metabolites_in_Rats_With_Irritable_Bowel_Syndrome_TIF/8197904
<p>Irritable bowel syndrome (IBS) is a common gastrointestinal dysfunctional disease. The pathophysiology of IBS is, however, largely unknown. This study aimed to determine whether evaluation of fecal metabolite and microbiota profiles may offer an opportunity to identify a novel pathophysiological target for IBS, and to reveal possible gut microbe–metabolite associations. By using gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and 16S rRNA gene sequencing, we measured fecal metabolites and microbiota of the control and water avoidance stress (WAS)-induced IBS rats. We found a significantly differential metabolite profile between the IBS and control groups; a cluster of metabolites was also found to be significantly associated with the amount of defecations. Moreover, the WAS group exhibited a decreased alpha diversity of the microbial population as compared to the control group. However, the characteristics of gut microbiota could not differentiate the IBS group from the control group. Correlation of the metabolite level with the number of microbial genera showed no significant association between the control and IBS groups. This study provides a global perspective on metabolomics and microbiota profiling in WAS-induced IBS model and a theoretical basis for research on the pathophysiology of IBS.</p>