10.3389/fphys.2019.00631.s001
Song Yang
Song
Yang
Jie Luo
Jie
Luo
Yalan Long
Yalan
Long
Jie Du
Jie
Du
GangChun Xu
GangChun
Xu
Liulan Zhao
Liulan
Zhao
Zongjun Du
Zongjun
Du
Wei Luo
Wei
Luo
Yan Wang
Yan
Wang
Zhi He
Zhi
He
Data_Sheet_1_Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing.doc
Frontiers
2019
rice-fish mode
diets
ecological aquaculture
animal welfare
microRNA sequencing
oxidative stress
2019-05-29 07:56:44
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Mixed_Diets_Reduce_the_Oxidative_Stress_of_Common_Carp_Cyprinus_carpio_Based_on_MicroRNA_Sequencing_doc/8197559
<p>The rice-fish mode, a mode of ecological aquaculture, has become a popular research topic in recent years. The antioxidant capacity of fish can be affected by the type of diet. Three groups of adult common carp (initial weight 517.8 ± 50 g) were fed earthworm (group A), earthworm + duckweed (group M), and duckweed (group P). The antioxidant capacity of common carp (Cyprinus carpio) was evaluated by histopathological sectioning, antioxidant enzyme activity, and the miRNA transcriptome profile. The pathological changes in group M were lighter than those in groups C and A. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) significantly increased in group M, and the malondialdehyde content (MDA) significantly decreased (p < 0.05). Additionally, nine differentially expressed miRNAs (DEMs) were found between groups A and M, and eight DEMs found between groups P and M were identified in the liver of common carp. Five miRNAs were reported to be related to oxidative stress, including miR-137-3p, miR-143-3p, miR-146a-5p, miR-21-5p, and miR-125b-5p. Compared with group M, all five detected miRNAs were upregulated in group A, and four of the detected miRNAs were upregulated in group P. The targets of the five miRNAs were further predicted via functional analysis. Our study confirmed that omnivorous common carp exhibits stronger antioxidant capacity when feeding on both an animal diet and a plant diet.</p>