10.3389/fphys.2019.00631.s001 Song Yang Song Yang Jie Luo Jie Luo Yalan Long Yalan Long Jie Du Jie Du GangChun Xu GangChun Xu Liulan Zhao Liulan Zhao Zongjun Du Zongjun Du Wei Luo Wei Luo Yan Wang Yan Wang Zhi He Zhi He Data_Sheet_1_Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing.doc Frontiers 2019 rice-fish mode diets ecological aquaculture animal welfare microRNA sequencing oxidative stress 2019-05-29 07:56:44 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Mixed_Diets_Reduce_the_Oxidative_Stress_of_Common_Carp_Cyprinus_carpio_Based_on_MicroRNA_Sequencing_doc/8197559 <p>The rice-fish mode, a mode of ecological aquaculture, has become a popular research topic in recent years. The antioxidant capacity of fish can be affected by the type of diet. Three groups of adult common carp (initial weight 517.8 ± 50 g) were fed earthworm (group A), earthworm + duckweed (group M), and duckweed (group P). The antioxidant capacity of common carp (Cyprinus carpio) was evaluated by histopathological sectioning, antioxidant enzyme activity, and the miRNA transcriptome profile. The pathological changes in group M were lighter than those in groups C and A. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) significantly increased in group M, and the malondialdehyde content (MDA) significantly decreased (p < 0.05). Additionally, nine differentially expressed miRNAs (DEMs) were found between groups A and M, and eight DEMs found between groups P and M were identified in the liver of common carp. Five miRNAs were reported to be related to oxidative stress, including miR-137-3p, miR-143-3p, miR-146a-5p, miR-21-5p, and miR-125b-5p. Compared with group M, all five detected miRNAs were upregulated in group A, and four of the detected miRNAs were upregulated in group P. The targets of the five miRNAs were further predicted via functional analysis. Our study confirmed that omnivorous common carp exhibits stronger antioxidant capacity when feeding on both an animal diet and a plant diet.</p>