10.3389/fimmu.2019.01216.s004
Laurent M. Paardekooper
Laurent M.
Paardekooper
Ilse Dingjan
Ilse
Dingjan
Peter T. A. Linders
Peter T. A.
Linders
Alexander H. J. Staal
Alexander H. J.
Staal
Simona M. Cristescu
Simona M.
Cristescu
Wilco C. E. P. Verberk
Wilco C. E. P.
Verberk
Geert van den Bogaart
Geert
van den Bogaart
Image_3_Human Monocyte-Derived Dendritic Cells Produce Millimolar Concentrations of ROS in Phagosomes Per Second.PNG
Frontiers
2019
dendritic cells (DCs)
reactive oxygen species (ROS)
phagosomes
quantitative biology
NOX2
2019-05-29 04:11:24
Figure
https://frontiersin.figshare.com/articles/figure/Image_3_Human_Monocyte-Derived_Dendritic_Cells_Produce_Millimolar_Concentrations_of_ROS_in_Phagosomes_Per_Second_PNG/8195519
<p>Neutrophils kill ingested pathogens by the so-called oxidative burst, where reactive oxygen species (ROS) are produced in the lumen of phagosomes at very high rates (mM/s), although these rates can only be maintained for a short period (minutes). In contrast, dendritic cells produce ROS at much lower rates, but they can sustain production for much longer after pathogen uptake (hours). It is becoming increasingly clear that this slow but prolonged ROS production is essential for antigen cross-presentation to activate cytolytic T cells, and for shaping the repertoire of antigen fragments for presentation to helper T cells. However, despite this importance of ROS production by dendritic cells for activation of the adaptive immune system, their actual ROS production rates have never been quantified. Here, we quantified ROS production in human monocyte-derived dendritic cells by measuring the oxygen consumption rate during phagocytosis. Although a large variation in oxygen consumption and phagocytic capacity was present among individuals and cells, we estimate a ROS production rate of on average ~0.5 mM/s per phagosome. Quantitative microscopy approaches showed that ROS is produced within minutes after pathogen encounter at the nascent phagocytic cup. H<sub>2</sub>DCFDA measurements revealed that ROS production is sustained for at least ~10 h after uptake. While ROS are produced by dendritic cells at an about 10-fold lower rate than by neutrophils, the net total ROS production is approximately similar. These are the first quantitative estimates of ROS production by a cell capable of antigen cross-presentation. Our findings provide a quantitative insight in how ROS affect dendritic cell function.</p>