10.3389/fmicb.2019.01016.s005
Yimei Huang
Yimei
Huang
Bin Li
Bin
Li
Jian Yin
Jian
Yin
Qiaosong Yang
Qiaosong
Yang
Ou Sheng
Ou
Sheng
Guiming Deng
Guiming
Deng
Chunyu Li
Chunyu
Li
Chunhua Hu
Chunhua
Hu
Tao Dong
Tao
Dong
Tongxin Dou
Tongxin
Dou
Huijun Gao
Huijun
Gao
Fangcheng Bi
Fangcheng
Bi
Ganjun Yi
Ganjun
Yi
Image_5_CgGCS, Encoding a Glucosylceramide Synthase, Is Required for Growth, Conidiation and Pathogenicity in Colletotrichum gloeosporioides.TIF
Frontiers
2019
Colletotrichum gloeosporioides
glucosylceramide
GCS conidiogenesis
growth rate
pathogenicity
2019-05-21 04:39:55
Figure
https://frontiersin.figshare.com/articles/figure/Image_5_CgGCS_Encoding_a_Glucosylceramide_Synthase_Is_Required_for_Growth_Conidiation_and_Pathogenicity_in_Colletotrichum_gloeosporioides_TIF/8157746
<p>Fungal glucosylceramide plays important role in cell division, hyphal formation and growth, spore germination and the modulation of virulence and has recently been considered as target for small molecule inhibitors. In this study, we characterized CgGCS, a protein encoding a glucosylceramide synthase (GCS) in Colletotrichum gloeosporioides. Disruption of CgGCS resulted in a severe reduction of mycelial growth and defects in conidiogenesis. Sphingolipid profile analysis revealed large decreases in glucosylceramide production in the mutant strains. Pathogenicity assays indicated that the ability of the ΔCgGCS mutants to invade both tomato and mango hosts was almost lost. In addition, the expression levels of many genes, especially those related to metabolism, were shown to be affected by the mutation of CgGCS via transcriptome analysis. Overall, our results demonstrate that C. gloeosporioides glucosylceramide is an important regulatory factor in fungal growth, conidiation, and pathogenesis in hosts.</p>