10.3389/fimmu.2019.00776.s002
Liang Jiang
Liang
Jiang
Weiqiang Liu
Weiqiang
Liu
Huizhen Guo
Huizhen
Guo
Yinghui Dang
Yinghui
Dang
Tingcai Cheng
Tingcai
Cheng
Wanying Yang
Wanying
Yang
Qiang Sun
Qiang
Sun
Bingbing Wang
Bingbing
Wang
Yumei Wang
Yumei
Wang
Enyu Xie
Enyu
Xie
Qingyou Xia
Qingyou
Xia
Table_1_Distinct Functions of Bombyx mori Peptidoglycan Recognition Protein 2 in Immune Responses to Bacteria and Viruses.xlsx
Frontiers
2019
DAP-type PGN
PGRP
Imd
silkworm
BmNPV
PTEN
Akt
apoptosis
2019-04-12 04:10:40
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_Distinct_Functions_of_Bombyx_mori_Peptidoglycan_Recognition_Protein_2_in_Immune_Responses_to_Bacteria_and_Viruses_xlsx/7986668
<p>Peptidoglycan recognition protein (PGRP) is an important pattern recognition receptor in innate immunity that is vital for bacterial recognition and defense in insects. Few studies report the role of PGRP in viral infection. Here we cloned two forms of PGRP from the model lepidopteran Bombyx mori: BmPGRP2-1 is a transmembrane protein, whereas BmPGRP2-2 is an intracellular protein. BmPGRP2-1 bound to diaminopimelic acid (DAP)-type peptidoglycan (PGN) to activate the canonical immune deficiency (Imd) pathway. BmPGRP2-2 knockdown reduced B. mori nucleopolyhedrovirus (BmNPV) multiplication and mortality in cell lines and in silkworm larvae, while its overexpression increased viral replication. Transcriptome and quantitative PCR (qPCR) results confirmed that BmPGRP2 negatively regulated phosphatase and tensin homolog (PTEN). BmPGRP2-2 expression was induced by BmNPV, and the protein suppressed PTEN-phosphoinositide 3-kinase (PI3K)/Akt signaling to inhibit cell apoptosis, suggesting that BmNPV modulates BmPGRP2-2-PTEN-PI3K/Akt signaling to evade host antiviral defense. These results demonstrate that the two forms of BmPGRP2 have different functions in host responses to bacteria and viruses.</p>