10.3389/fsurg.2019.00013.s003 Jessica C. Papali'i-Curtin Jessica C. Papali'i-Curtin Helen D. Brasch Helen D. Brasch Bede van Schaijik Bede van Schaijik Jennifer de Jongh Jennifer de Jongh Reginald W. Marsh Reginald W. Marsh Swee T. Tan Swee T. Tan Tinte Itinteang Tinte Itinteang Image_3_Expression of Components of the Renin-Angiotensin System in Pyogenic Granuloma.jpg Frontiers 2019 pyogenic granuloma vascular tumor renin-angiotensin system embryonic stem cells angiotensin converting enzyme 2019-04-09 07:29:49 Figure https://frontiersin.figshare.com/articles/figure/Image_3_Expression_of_Components_of_the_Renin-Angiotensin_System_in_Pyogenic_Granuloma_jpg/7969487 <p>Background: There is a growing body of research demonstrating expression of the renin-angiotensin system (RAS) by a putative embryonic stem cell (ESC)-like population within vascular anomalies. This study investigated the expression of components of the RAS in relation to the putative ESC-like population within pyogenic granuloma (PG) that we have recently reported.</p><p>Methods: PG samples from 14 patients were analyzed for the expression of components of the RAS: pro-renin receptor (PRR), angiotensin converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1) and angiotensin II receptor 2 (ATIIR2), using 3,3-diaminobenzidine (DAB) immunohistochemical (IHC) staining. Immunofluorescence (IF) IHC staining was performed to localize these proteins on four of the PG samples. RT-qPCR was performed on two snap-frozen PG samples. Western blotting (WB) was performed on one snap-frozen PG sample and two PG-derived primary cell lines.</p><p>Results: DAB IHC staining demonstrated the expression of ACE, PRR, ATIIR1, and ATIIR2 in all 14 PG tissue samples. RT-qPCR analysis confirmed abundant mRNA transcripts for PRR, ACE, AIITR1 and ATIIR2, relative to the housekeeping gene. WB confirmed the presence of PRR, ATIIR1, and ACE in the PG tissue sample, and PRR and ATIIR1, in the PG-derived primary cell lines. IF IHC staining demonstrated the expression of PRR, ACE, ATIIR1 on the primitive population that expressed NANOG and SOX2 on the ERG<sup>+</sup> endothelium of the microvessels within PG.</p><p>Conclusion: We have demonstrated the expression of PRR, ACE, and ATIIR1 by the putative the ESC-like population within PG.</p>