10.3389/fpls.2019.00417.s010
Zhaowu Li
Zhaowu
Li
Jianzhong Huang
Jianzhong
Huang
Zhangying Wang
Zhangying
Wang
Fen Meng
Fen
Meng
Siyuan Zhang
Siyuan
Zhang
Xiaoqiu Wu
Xiaoqiu
Wu
Zhihong Zhang
Zhihong
Zhang
Zhiyong Gao
Zhiyong
Gao
Table_6_Overexpression of Arabidopsis Nucleotide-Binding and Leucine-Rich Repeat Genes RPS2 and RPM1(D505V) Confers Broad-Spectrum Disease Resistance in Rice.XLSX
Frontiers
2019
AtRPS2
AtRPM1
rice
Magnaporthe oryzae
Xanthomonas oryzae
Nilaparvata lugens
2019-04-05 04:25:14
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_6_Overexpression_of_Arabidopsis_Nucleotide-Binding_and_Leucine-Rich_Repeat_Genes_RPS2_and_RPM1_D505V_Confers_Broad-Spectrum_Disease_Resistance_in_Rice_XLSX/7956998
<p>The nucleotide-binding domain leucine-rich repeat (NLR) immune receptors play important roles in innate plant immunity. The activation of NLRs is specifically induced by their cognate effectors released from pathogens. Autoactive NLRs are expected to confer broad-spectrum resistance because they do not need cognate effectors to activate their immune responses. In this study, we demonstrated that the NLR genes RPS2 and RPM1(D505V) from Arabidopsis were autoactive in Oryza sativa and conferred broad-spectrum resistance to fungal pathogen Magnaporthe oryzae, bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), and pest brown planthopper (BPH, Nilaparvata lugens Stål). These results revealed that interfamily transfer of dicot NLRs to monocot species could be functional. The transgenic plants displayed early and strong induction of reactive oxygen species (ROS), callose deposition, and expression of defense-related genes after challenged with M. oryzae. The transcriptome analysis showed that the expressions of some defense-related genes were primed to adapt the transformed autoactive NLRs in the transgenic plants. This study indicates that autoactive NLRs are a promising resource for breeding crops with broad-spectrum resistance and provides new insights for engineering disease resistance.</p>