10.3389/fimmu.2019.00408.s005 Celestine N. Wanjalla Celestine N. Wanjalla Wyatt J. McDonnell Wyatt J. McDonnell Louise Barnett Louise Barnett Joshua D. Simmons Joshua D. Simmons Briana D. Furch Briana D. Furch Morgan C. Lima Morgan C. Lima Beverly O. Woodward Beverly O. Woodward Run Fan Run Fan Ye Fei Ye Fei Paxton G. Baker Paxton G. Baker Ramesh Ram Ramesh Ram Mark A. Pilkinton Mark A. Pilkinton Mona Mashayekhi Mona Mashayekhi Nancy J. Brown Nancy J. Brown Simon A. Mallal Simon A. Mallal Spyros A. Kalams Spyros A. Kalams John R. Koethe John R. Koethe Image_2_Adipose Tissue in Persons With HIV Is Enriched for CD4+ T Effector Memory and T Effector Memory RA+ Cells, Which Show Higher CD69 Expression and CD57, CX3CR1, GPR56 Co-expression With Increasing Glucose Intolerance.TIFF Frontiers 2019 HIV—human immunodeficiency virus adipose tissue diabetes mellitus TEMRA T effector memory cells GPR56 CX3CR1 memory T cells 2019-03-19 04:44:30 Figure https://frontiersin.figshare.com/articles/figure/Image_2_Adipose_Tissue_in_Persons_With_HIV_Is_Enriched_for_CD4_T_Effector_Memory_and_T_Effector_Memory_RA_Cells_Which_Show_Higher_CD69_Expression_and_CD57_CX3CR1_GPR56_Co-expression_With_Increasing_Glucose_Intolerance_TIFF/7860560 <p>Chronic T cell activation and accelerated immune senescence are hallmarks of HIV infection, which may contribute to the increased risk of cardiometabolic diseases in people living with HIV (PLWH). T lymphocytes play a central role in modulating adipose tissue inflammation and, by extension, adipocyte energy storage and release. Here, we assessed the CD4<sup>+</sup> and CD8<sup>+</sup> T cell profiles in the subcutaneous adipose tissue (SAT) and blood of non-diabetic (n = 9; fasting blood glucose [FBG] < 100 mg/dL), pre-diabetic (n = 8; FBG = 100–125 mg/dL) and diabetic (n = 9; FBG ≥ 126 mg/dL) PLWH, in addition to non- and pre-diabetic, HIV-negative controls (n = 8). SAT was collected by liposuction and T cells were extracted by collagenase digestion. The proportion of naïve (T<sub>Nai</sub>) CD45RO<sup>−</sup>CCR7<sup>+</sup>, effector memory (T<sub>EM</sub>) CD45RO<sup>+</sup>CCR7<sup>−</sup>, central memory (T<sub>CM</sub>) CD45RO<sup>+</sup>CCR7<sup>+</sup>, and effector memory revertant RA<sup>+</sup>(T<sub>EMRA</sub>) CD45RO<sup>−</sup>CCR7<sup>−</sup> CD4<sup>+</sup> and CD8<sup>+</sup> T cells were measured by flow cytometry. CD4<sup>+</sup> and CD8<sup>+</sup> T<sub>EM</sub> and T<sub>EMRA</sub> were significantly enriched in SAT of PLWH compared to blood. The proportions of SAT CD4<sup>+</sup> and CD8<sup>+</sup> memory subsets were similar across metabolic status categories in the PLWH, but CD4<sup>+</sup> T cell expression of the CD69 early-activation and tissue residence marker, particularly on T<sub>EM</sub> cells, increased with progressive glucose intolerance. Use of t-distributed Stochastic Neighbor Embedding (t-SNE) identified a separate group of predominantly CD69<sup>lo</sup> T<sub>EM</sub> and T<sub>EMRA</sub> cells co-expressing CD57, CX<sub>3</sub>CR1, and GPR56, which were significantly greater in diabetics compared to non-diabetics. Expression of the CX<sub>3</sub>CR1 and GPR56 markers indicate these T<sub>EM</sub> and T<sub>EMRA</sub> cells may have anti-viral specificity. Compared to HIV-negative controls, SAT from PLWH had an increased CD8:CD4 ratio, but the distribution of CD4<sup>+</sup> and CD8<sup>+</sup> memory subsets was similar irrespective of HIV status. Finally, whole adipose tissue from PLWH had significantly higher expression of TLR2, TLR8, and multiple chemokines potentially relevant to immune cell homing compared to HIV-negative controls with similar glucose tolerance.</p>