10.3389/fmicb.2016.01481.s001
Marta Irla
Marta
Irla
Tonje M. B. Heggeset
Tonje
M. B. Heggeset
Ingemar Nærdal
Ingemar
Nærdal
Lidia Paul
Lidia
Paul
Tone Haugen
Tone
Haugen
Simone B. Le
Simone B.
Le
Trygve Brautaset
Trygve
Brautaset
Volker F. Wendisch
Volker F.
Wendisch
Data_Sheet_1_Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production.PDF
Frontiers
2019
Bacillus methanolicus
thermophile
methylotroph
genetic tool box
theta-replicating plasmids
gene expression
2019-03-12 12:01:06
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Genome-Based_Genetic_Tool_Development_for_Bacillus_methanolicus_Theta-_and_Rolling_Circle-Replicating_Plasmids_for_Inducible_Gene_Expression_and_Application_to_Methanol-Based_Cadaverine_Production_PDF/7831262
<p>Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter and a putative mannitol activator gene mtlR were identified. The mannitol inducible promoter was applied for controlled gene expression using fluorescent reporter proteins and a flow cytometry analysis, and improved by changing the -35 promoter region and by co-expression of the mtlR regulator gene. For independent complementary gene expression control, the heterologous xylose-inducible system from B. megaterium was employed and a two-plasmid gene expression system was developed. Four different replicons for expression vectors were compared with respect to their copy number and stability. As an application example, methanol-based production of cadaverine was shown to be improved from 6.5 to 10.2 g/L when a heterologous lysine decarboxylase gene cadA was expressed from a theta-replicating rather than a rolling-circle replicating vector. The current work on inducible promoter systems and compatible theta- or rolling circle-replicating vectors is an important extension of the poorly developed B. methanolicus genetic toolbox, valuable for genetic engineering and further exploration of this bacterium.</p>