10.3389/fpls.2019.00218.s004
Gaurav Sablok
Gaurav
Sablok
Ali Amiryousefi
Ali
Amiryousefi
Xiaolan He
Xiaolan
He
Jaakko Hyvönen
Jaakko
Hyvönen
Péter Poczai
Péter
Poczai
Image_3_Sequencing the Plastid Genome of Giant Ragweed (Ambrosia trifida, Asteraceae) From a Herbarium Specimen.pdf
Frontiers
2019
Ambrosia trifida
chloroplast genome
genome skimming
glyphosate
herbicide resistance
invasive plants
noxious weeds
ragweed
2019-02-28 04:32:06
Figure
https://frontiersin.figshare.com/articles/figure/Image_3_Sequencing_the_Plastid_Genome_of_Giant_Ragweed_Ambrosia_trifida_Asteraceae_From_a_Herbarium_Specimen_pdf/7781555
<p>We report the first plastome sequence of giant ragweed (Ambrosia trifida); with this new genome information, we assessed the phylogeny of Asteraceae and the transcriptional profiling against glyphosate resistance in giant ragweed. Assembly and genic features show a normal angiosperm quadripartite plastome structure with no signatures of deviation in gene directionality. Comparative analysis revealed large inversions across the plastome of giant ragweed and the previously sequenced members of the plant family. Asteraceae plastid genomes contain two inversions of 22.8 and 3.3 kb; the former is located between trnS-GCU and trnG-UCC genes, and the latter between trnE-UUC and trnT-GGU genes. The plastid genome sequences of A. trifida and the related species, Ambrosia artemisiifolia, are identical in gene content and arrangement, but they differ in length. The phylogeny is well-resolved and congruent with previous hypotheses about the phylogenetic relationship of Asteraceae. Transcriptomic analysis revealed divergence in the relative expressions at the exonic and intronic levels, providing hints toward the ecological adaptation of the genus. Giant ragweed shows various levels of glyphosate resistance, with introns displaying higher expression patterns at resistant time points after the assumed herbicide treatment.</p>