10.3389/fmicb.2019.00383.s004
Svetlana Lockwood
Svetlana
Lockwood
Kelly A. Brayton
Kelly
A. Brayton
Jeff A. Daily
Jeff A.
Daily
Shira L. Broschat
Shira L.
Broschat
Table_2_Whole Proteome Clustering of 2,307 Proteobacterial Genomes Reveals Conserved Proteins and Significant Annotation Issues.xlsx
Frontiers
2019
whole proteome clustering
Proteobacterial genomes
conserved proteins
misannotations
annotation errors
minimal genome
protein sequence clustering
essential proteins
2019-02-28 04:29:13
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_2_Whole_Proteome_Clustering_of_2_307_Proteobacterial_Genomes_Reveals_Conserved_Proteins_and_Significant_Annotation_Issues_xlsx/7781489
<p>We clustered 8.76 M protein sequences deduced from 2,307 completely sequenced Proteobacterial genomes resulting in 707,311 clusters of one or more sequences of which 224,442 ranged in size from 2 to 2,894 sequences. To our knowledge this is the first study of this scale. We were surprised to find that no single cluster contained a representative sequence from all the organisms in the study. Given the minimal genome concept, we expected to find a shared set of proteins. To determine why the clusters did not have universal representation we chose four essential proteins, the chaperonin GroEL, DNA dependent RNA polymerase subunits beta and beta′ (RpoB/RpoB′), and DNA polymerase I (PolA), representing fundamental cellular functions, and examined their cluster distribution. We found these proteins to be remarkably conserved with certain caveats. Although the groEL gene was universally conserved in all the organisms in the study, the protein was not represented in all the deduced proteomes. The genes for RpoB and RpoB′ were missing from two genomes and merged in 88, and the sequences were sufficiently divergent that they formed separate clusters for 18 RpoB proteins (seven clusters) and 14 RpoB′ proteins (three clusters). For PolA, 52 organisms lacked an identifiable sequence, and seven sequences were sufficiently divergent that they formed five separate clusters. Interestingly, organisms lacking an identifiable PolA and those with divergent RpoB/RpoB′ were predominantly endosymbionts. Furthermore, we present a range of examples of annotation issues that caused the deduced proteins to be incorrectly represented in the proteome. These annotation issues made our task of determining protein conservation more difficult than expected and also represent a significant obstacle for high-throughput analyses.</p>