Chen, Wei Sheng, Zhonghua Cai, Yicong Li, Qianlong Wei, Xiangjin Xie, Lihong Jiao, Guiai Shao, Gaoneng Tang, Shaoqing Wang, Jianlong Hu, Peisong Data_Sheet_1_Rice Morphogenesis and Chlorophyll Accumulation Is Regulated by the Protein Encoded by NRL3 and Its Interaction With NAL9.pdf <p>Rice yield is closely related to plant leaf shape and chlorophyll content. In this study, we isolated and identified a narrow and rolled leaf mutant, temporarily named nrl3 with darker green leaves. Histological analysis showed that nrl3 has a reduced number of vascular bundles and undergoes abnormal abaxial sclerenchymatous cell differentiation. The NRL3 mutant phenotype was controlled by a single recessive gene, fine-mapped to a 221 kb interval between Indel3 and RM2322 on Chr3. There are 42 ORF in this interval. Sequencing identified an SNP mutant leading to a premature stop in ORF 18, the candidate gene. Bioinformation analysis indicated that NRL3 encodes a novel protein with unknown function. NRL3 is localized in cytoplasm, membrane and nucleus. Expression analysis of nrl3 showed that genes involved in chlorophyll synthesis were significantly up-regulated while those involved in chlorophyll degradation and programmed cell death (PCD) were significantly down-regulated. The expression levels of photosynthesis genes were also affected. Y2H and BIFC assays indicated that NRL3 interacts directly with NAL9/VYL to regulate leaf morphology in rice. Thus, NRL3 plays an important role in leaf morphogenesis and chlorophyll accumulation, and can be used as a new gene resource for constructing improved rice.</p> nrl3;narrow and rolled leaf;leaf morphogenesis;chlorophyll accumulation;rice (Oryza sativa L.);improvement 2019-02-19
    https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Rice_Morphogenesis_and_Chlorophyll_Accumulation_Is_Regulated_by_the_Protein_Encoded_by_NRL3_and_Its_Interaction_With_NAL9_pdf/7739273
10.3389/fpls.2019.00175.s001