%0 Generic %A Chen, Wei %A Sheng, Zhonghua %A Cai, Yicong %A Li, Qianlong %A Wei, Xiangjin %A Xie, Lihong %A Jiao, Guiai %A Shao, Gaoneng %A Tang, Shaoqing %A Wang, Jianlong %A Hu, Peisong %D 2019 %T Data_Sheet_1_Rice Morphogenesis and Chlorophyll Accumulation Is Regulated by the Protein Encoded by NRL3 and Its Interaction With NAL9.pdf %U https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Rice_Morphogenesis_and_Chlorophyll_Accumulation_Is_Regulated_by_the_Protein_Encoded_by_NRL3_and_Its_Interaction_With_NAL9_pdf/7739273 %R 10.3389/fpls.2019.00175.s001 %2 https://frontiersin.figshare.com/ndownloader/files/14400146 %K nrl3 %K narrow and rolled leaf %K leaf morphogenesis %K chlorophyll accumulation %K rice (Oryza sativa L.) %K improvement %X

Rice yield is closely related to plant leaf shape and chlorophyll content. In this study, we isolated and identified a narrow and rolled leaf mutant, temporarily named nrl3 with darker green leaves. Histological analysis showed that nrl3 has a reduced number of vascular bundles and undergoes abnormal abaxial sclerenchymatous cell differentiation. The NRL3 mutant phenotype was controlled by a single recessive gene, fine-mapped to a 221 kb interval between Indel3 and RM2322 on Chr3. There are 42 ORF in this interval. Sequencing identified an SNP mutant leading to a premature stop in ORF 18, the candidate gene. Bioinformation analysis indicated that NRL3 encodes a novel protein with unknown function. NRL3 is localized in cytoplasm, membrane and nucleus. Expression analysis of nrl3 showed that genes involved in chlorophyll synthesis were significantly up-regulated while those involved in chlorophyll degradation and programmed cell death (PCD) were significantly down-regulated. The expression levels of photosynthesis genes were also affected. Y2H and BIFC assays indicated that NRL3 interacts directly with NAL9/VYL to regulate leaf morphology in rice. Thus, NRL3 plays an important role in leaf morphogenesis and chlorophyll accumulation, and can be used as a new gene resource for constructing improved rice.

%I Frontiers