Xu, Tianhao Keller, Ashleigh J. Martinez, Gustavo Table_1_NFAT1 and NFAT2 Differentially Regulate CTL Differentiation Upon Acute Viral Infection.xlsx <p>CD8<sup>+</sup> T cell differentiation orchestrated by transcription regulators is critical for balancing pathogen eradication and long-term immunity by effector and memory CTLs, respectively. The transcription factor Nuclear Factor of Activated T cells (NFAT) family members are known for their roles in T cell development and activation but still largely undetermined in CD8<sup>+</sup> T cell differentiation in vivo. Here, we interrogated the role of two NFAT family members, NFAT1 and NFAT2, in the effector and memory phase of CD8<sup>+</sup> T cell differentiation using LCMV<sup>Arm</sup> acute infection model. We found that NFAT1 is critical for effector population generation whereas NFAT2 is required for promoting memory CTLs in a cell intrinsic manner. Moreover, we found that mice lacking both NFAT1 and NFAT2 in T cells display a significant increase in KLRG1<sup>hi</sup> CD127<sup>hi</sup> population and are unable to clear an acute viral infection. NFAT-deficient CTLs showed different degrees of impaired IFN-γ and TNF-α expression with NFAT1 being mainly responsible for IFN-γ production upon ex-vivo stimulation as well as for antigen-specific cytotoxicity. Our results suggest that NFAT1 and NFAT2 have distinct roles in mediating CD8<sup>+</sup> T cell differentiation and function.</p> NFAT1;NFAT2;CD8+ T cells;differentiation;effector;memory;LCMV 2019-02-15
    https://frontiersin.figshare.com/articles/dataset/Table_1_NFAT1_and_NFAT2_Differentially_Regulate_CTL_Differentiation_Upon_Acute_Viral_Infection_xlsx/7723559
10.3389/fimmu.2019.00184.s002