10.3389/fncel.2019.00039.s001 Peter Quicke Peter Quicke Chenchen Song Chenchen Song Eric J. McKimm Eric J. McKimm Milena M. Milosevic Milena M. Milosevic Carmel L. Howe Carmel L. Howe Mark Neil Mark Neil Simon R. Schultz Simon R. Schultz Srdjan D. Antic Srdjan D. Antic Amanda J. Foust Amanda J. Foust Thomas Knöpfel Thomas Knöpfel Data_Sheet_1_Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators.pdf Frontiers 2019 voltage imaging cerebral cortex sparse expression optogenetics transgenic 2019-02-14 04:10:33 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Single-Neuron_Level_One-Photon_Voltage_Imaging_With_Sparsely_Targeted_Genetically_Encoded_Voltage_Indicators_pdf/7716767 <p>Voltage imaging of many neurons simultaneously at single-cell resolution is hampered by the difficulty of detecting small voltage signals from overlapping neuronal processes in neural tissue. Recent advances in genetically encoded voltage indicator (GEVI) imaging have shown single-cell resolution optical voltage recordings in intact tissue through imaging naturally sparse cell classes, sparse viral expression, soma restricted expression, advanced optical systems, or a combination of these. Widespread sparse and strong transgenic GEVI expression would enable straightforward optical access to a densely occurring cell type, such as cortical pyramidal cells. Here we demonstrate that a recently described sparse transgenic expression strategy can enable single-cell resolution voltage imaging of cortical pyramidal cells in intact brain tissue without restricting expression to the soma. We also quantify the functional crosstalk in brain tissue and discuss optimal imaging rates to inform future GEVI experimental design.</p>