Table_3_Individualization of Hematopoietic Stem Cell Transplantation Using Alpha/Beta T-Cell Depletion.pdf Emelie Rådestad Mikael Sundin Johan Törlén Sarah Thunberg Björn Önfelt Per Ljungman Emma Watz Jonas Mattsson Michael Uhlin 10.3389/fimmu.2019.00189.s003 https://frontiersin.figshare.com/articles/dataset/Table_3_Individualization_of_Hematopoietic_Stem_Cell_Transplantation_Using_Alpha_Beta_T-Cell_Depletion_pdf/7700345 <p>Allogeneic hematopoietic stem cell transplantation (HSCT) is associated with several potentially lethal complications. Higher levels of CD3+ T-cells in the graft have been associated with increased risk of graft-versus-host disease (GVHD), but also beneficial graft-versus-leukemia effect and reduced infections. To tackle post-transplant complications, donor lymphocyte infusions have been used but with an increased risk of GVHD. To reduce this risk, we performed depletion of αβ T-cells and treated 12 patients post-HSCT suffering from infections and/or poor immune reconstitution. The αβ T-cell depleted cell products were characterized by flow cytometry. The median log depletion of αβ T-cells was −4.3 and the median yield of γδ T-cells was 73.5%. The median CD34+ cell dose was 4.4 × 10<sup>6</sup>/kg. All 12 patients were alive 3 months after infusion and after 1 year, two patients had died. No infusion-related side effects were reported and no severe acute GVHD (grade III-IV) developed in any patient post-infusion. Overall, 3 months after infusion 11 out of 12 patients had increased levels of platelets and/or granulocytes. In conclusion, we describe the use of αβ T-cell depleted products as stem cell boosters with encouraging results.</p> 2019-02-11 04:26:02 αβ T-cell depletion γδ T-cells allogeneic hematopoietic stem cell transplantation stem cell booster donor lymphocyte infusion graft manipulation CliniMACS