10.3389/fcimb.2019.00014.s002 Kristine M. Wylie Kristine M. Wylie Todd N. Wylie Todd N. Wylie Patrick J. Minx Patrick J. Minx David A. Rosen David A. Rosen Table_2_Whole-Genome Sequencing of Klebsiella pneumoniae Isolates to Track Strain Progression in a Single Patient With Recurrent Urinary Tract Infection.XLSX Frontiers 2019 Klebsiella pneumoniae whole-genome sequencing single nucleotide variants urinary tract infection strain tracking 2019-02-08 04:02:46 Dataset https://frontiersin.figshare.com/articles/dataset/Table_2_Whole-Genome_Sequencing_of_Klebsiella_pneumoniae_Isolates_to_Track_Strain_Progression_in_a_Single_Patient_With_Recurrent_Urinary_Tract_Infection_XLSX/7692563 <p>Klebsiella pneumoniae is an important uropathogen that increasingly harbors broad-spectrum antibiotic resistance determinants. Evidence suggests that some same-strain recurrences in women with frequent urinary tract infections (UTIs) may emanate from a persistent intravesicular reservoir. Our objective was to analyze K. pneumoniae isolates collected over weeks from multiple body sites of a single patient with recurrent UTI in order to track ordered strain progression across body sites, as has been employed across patients in outbreak settings. Whole-genome sequencing of 26 K. pneumoniae isolates was performed utilizing the Illumina platform. PacBio sequencing was used to create a refined reference genome of the original urinary isolate (TOP52). Sequence variation was evaluated by comparing the 26 isolate sequences to the reference genome sequence. Whole-genome sequencing of the K. pneumoniae isolates from six different body sites of this patient with recurrent UTI demonstrated 100% chromosomal sequence identity of the isolates, with only a small P2 plasmid deletion in a minority of isolates. No single nucleotide variants were detected. The complete absence of single-nucleotide variants from 26 K. pneumoniae isolates from multiple body sites collected over weeks from a patient with recurrent UTI suggests that, unlike in an outbreak situation with strains collected from numerous patients, other methods are necessary to discern strain progression within a single host over a relatively short time frame.</p>