10.3389/fpls.2019.00064.s003
Fangzhou Liu
Fangzhou
Liu
Bin Yang
Bin
Yang
Aihong Zhang
Aihong
Zhang
Derong Ding
Derong
Ding
Guirong Wang
Guirong
Wang
Image_2_Plant-Mediated RNAi for Controlling Apolygus lucorum.jpeg
Frontiers
2019
RNA interference
transgenic plants
non-Bt
lethal gene
Apolygus lucorum
2019-02-06 04:26:20
Figure
https://frontiersin.figshare.com/articles/figure/Image_2_Plant-Mediated_RNAi_for_Controlling_Apolygus_lucorum_jpeg/7679078
<p>The polyphagous mirid bug Apolygus lucorum (Heteroptera: Miridae) is a serious pest of agricultural crops in China, with more than 200 species of host plants including two very important crops, maize and soybean. Currently, prevention and control of A. lucorum rely mainly on chemical pesticides that cause environmental as well as health related problems. Plant-mediated RNAi has proven to offer great potential for pest control in the past decade. In this study, we screened and obtained seven candidate genes (Alucβ-actin, AlucV-ATPase-A/D/E, AlucEif5A, AlucEcR-A, AlucIAP) by injection-based RNAi which produced A. lucorum mortality rates of 46.01–82.32% at day 7 after injection. Among them, the plant-mediated RNAi of AlucV-ATPase-E was successfully introduced into transgenic maize and soybean, and the populations of A. lucorum were significantly decreased following feeding on the transgenic maize and soybean. These results are intended to provide helpful insight into the generation of bug-resistant plants through a plant-mediated RNAi strategy.</p>