10.3389/fimmu.2019.00054.s002
Paul David
Paul
David
Dominik A. Megger
Dominik A.
Megger
Tamara Kaiser
Tamara
Kaiser
Tanja Werner
Tanja
Werner
Jia Liu
Jia
Liu
Lieping Chen
Lieping
Chen
Barbara Sitek
Barbara
Sitek
Ulf Dittmer
Ulf
Dittmer
Gennadiy Zelinskyy
Gennadiy
Zelinskyy
Table_2_The PD-1/PD-L1 Pathway Affects the Expansion and Function of Cytotoxic CD8+ T Cells During an Acute Retroviral Infection.xlsx
Frontiers
2019
CD8 T cells
PD-1
PD-L1
retrovirus
caspase 3
apoptosis
immunoregulation
2019-02-05 04:10:22
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_2_The_PD-1_PD-L1_Pathway_Affects_the_Expansion_and_Function_of_Cytotoxic_CD8_T_Cells_During_an_Acute_Retroviral_Infection_xlsx/7671248
<p>Cytotoxic CD8<sup>+</sup> T lymphocytes (CTL) efficiently control acute virus infections but can become exhausted when a chronic infection develops. The checkpoint receptor PD-1 suppresses the functionality of virus-specific CD8<sup>+</sup> T cells during chronic infection. However, the role of the PD-L1/PD-1 pathway during the acute phase of infections has not been well characterized. In the current study the effects of PD-1 or PD-L1 deficiency on the CD8<sup>+</sup> T cell response against Friend retroviral (FV) infection of knockout mice was analyzed during acute infection. We observed an enhanced proliferation, functional maturation, and reduced apoptosis of effector CD8<sup>+</sup> T cells in the absence of PD-1 or PD-L1. The knockout of PD-L1 had a stronger effect on the functionality of CD8<sup>+</sup> T cells than that of PD-1. Augmented CTL responses were associated with an improved control of FV replication. The strong phenotype of FV-infected PD-L1 knockout mice was independent of the interaction with CD80 as an additional receptor for PD-L1. Furthermore, we performed a detailed analysis of the production of different granzymes in virus-specific CD8<sup>+</sup> T cells and observed that especially the simultaneous production of multiple granzymes in individual T cells (multifunctionality) was under the control of the PD-1/PD-L1 pathway. The findings from this study allow for a better understanding of the development of antiviral cytotoxic immunity during acute viral infections.</p>