David, Paul Megger, Dominik A. Kaiser, Tamara Werner, Tanja Liu, Jia Chen, Lieping Sitek, Barbara Dittmer, Ulf Zelinskyy, Gennadiy Table_1_The PD-1/PD-L1 Pathway Affects the Expansion and Function of Cytotoxic CD8+ T Cells During an Acute Retroviral Infection.xlsx <p>Cytotoxic CD8<sup>+</sup> T lymphocytes (CTL) efficiently control acute virus infections but can become exhausted when a chronic infection develops. The checkpoint receptor PD-1 suppresses the functionality of virus-specific CD8<sup>+</sup> T cells during chronic infection. However, the role of the PD-L1/PD-1 pathway during the acute phase of infections has not been well characterized. In the current study the effects of PD-1 or PD-L1 deficiency on the CD8<sup>+</sup> T cell response against Friend retroviral (FV) infection of knockout mice was analyzed during acute infection. We observed an enhanced proliferation, functional maturation, and reduced apoptosis of effector CD8<sup>+</sup> T cells in the absence of PD-1 or PD-L1. The knockout of PD-L1 had a stronger effect on the functionality of CD8<sup>+</sup> T cells than that of PD-1. Augmented CTL responses were associated with an improved control of FV replication. The strong phenotype of FV-infected PD-L1 knockout mice was independent of the interaction with CD80 as an additional receptor for PD-L1. Furthermore, we performed a detailed analysis of the production of different granzymes in virus-specific CD8<sup>+</sup> T cells and observed that especially the simultaneous production of multiple granzymes in individual T cells (multifunctionality) was under the control of the PD-1/PD-L1 pathway. The findings from this study allow for a better understanding of the development of antiviral cytotoxic immunity during acute viral infections.</p> CD8 T cells;PD-1;PD-L1;retrovirus;caspase 3;apoptosis;immunoregulation 2019-02-05
    https://frontiersin.figshare.com/articles/dataset/Table_1_The_PD-1_PD-L1_Pathway_Affects_the_Expansion_and_Function_of_Cytotoxic_CD8_T_Cells_During_an_Acute_Retroviral_Infection_xlsx/7671245
10.3389/fimmu.2019.00054.s001