10.3389/fcimb.2019.00006.s005
Xianping Li
Xianping
Li
Liqiong Song
Liqiong
Song
Siyi Zhu
Siyi
Zhu
Yuchun Xiao
Yuchun
Xiao
Yuanming Huang
Yuanming
Huang
Yuting Hua
Yuting
Hua
Qiongfang Chu
Qiongfang
Chu
Zhihong Ren
Zhihong
Ren
Table_1_Two Strains of Lactobacilli Effectively Decrease the Colonization of VRE in a Mouse Model.xlsx
Frontiers
2019
Lactobacillus
Vancomycin-resistant Enterococcus
fecal microbiota transplantation
16S rRNA
RNA-seq
2019-01-30 04:05:50
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_Two_Strains_of_Lactobacilli_Effectively_Decrease_the_Colonization_of_VRE_in_a_Mouse_Model_xlsx/7647611
<p>Vancomycin-resistant Enterococcus (VRE) infection is a serious challenge for clinical management and there is no effective treatment at present. Fecal microbiota transplantation (FMT) and probiotic intervention have been shown to be promising approaches for reducing the colonization of certain pathogenic bacteria in the gastrointestinal tract, however, no such studies have been done on VRE. In this study, we evaluated the effect of FMT and two Lactobacillus strains (Y74 and HT121) on the colonization of VRE in a VRE-infection mouse model. We found that both Lactobacilli strains reduced VRE colonization rapidly. Fecal microbiota and colon mRNA expression analyses further showed that mice in FMT and the two Lactobacilli treatment groups restored their intestinal microbiota diversity faster than those in the phosphate buffer saline (PBS) treated group. Administration of Lactobacilli restored Firmicutes more quickly to the normal level, compared to FMT or PBS treatment, but restored Bacteroides to their normal level less quickly than FMT did. Furthermore, these treatments also had an impact on the relative abundance of intestinal microbiota composition from phylum to species level. RNA-seq showed that FMT treatment induced the expression of more genes in the colon, compared to the Lactobacilli treatment. Defense-related genes such as defensin α, Apoa1, and RegIII were down-regulated in both FMT and the two Lactobacilli treatment groups. Taken together, our findings indicate that both FMT and Lactobacilli treatments were effective in decreasing the colonization of VRE in the gut.</p>