10.3389/fnmol.2018.00323.s001
Alfredo Bellon
Alfredo
Bellon
Amelie Wegener
Amelie
Wegener
Adam R. Lescallette
Adam R.
Lescallette
Michael Valente
Michael
Valente
Seung-Kwon Yang
Seung-Kwon
Yang
Robert Gardette
Robert
Gardette
Julien Matricon
Julien
Matricon
Faycal Mouaffak
Faycal
Mouaffak
Paula Watts
Paula
Watts
Lene Vimeux
Lene
Vimeux
Jong K. Yun
Jong K.
Yun
Yuka Imamura Kawasawa
Yuka
Imamura Kawasawa
Gary A. Clawson
Gary A.
Clawson
Elisabeta Blandin
Elisabeta
Blandin
Boris Chaumette
Boris
Chaumette
Therese M. Jay
Therese M.
Jay
Marie-Odile Krebs
Marie-Odile
Krebs
Vincent Feuillet
Vincent
Feuillet
Anne Hosmalin
Anne
Hosmalin
Data_Sheet_1_Transdifferentiation of Human Circulating Monocytes Into Neuronal-Like Cells in 20 Days and Without Reprograming.pdf
Frontiers
2019
stem cells
dopamine
schizophrenia
neurite
in vitro model
GABA
neurodevelopment
autism
2019-01-24 09:09:47
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Transdifferentiation_of_Human_Circulating_Monocytes_Into_Neuronal-Like_Cells_in_20_Days_and_Without_Reprograming_pdf/7623815
<p>Despite progress, our understanding of psychiatric and neurological illnesses remains poor, at least in part due to the inability to access neurons directly from patients. Currently, there are in vitro models available but significant work remains, including the search for a less invasive, inexpensive and rapid method to obtain neuronal-like cells with the capacity to deliver reproducible results. Here, we present a new protocol to transdifferentiate human circulating monocytes into neuronal-like cells in 20 days and without the need for viral insertion or reprograming. We have thoroughly characterized these monocyte-derived-neuronal-like cells (MDNCs) through various approaches including immunofluorescence (IF), flow cytometry, qRT-PCR, single cell mRNA sequencing, electrophysiology and pharmacological techniques. These MDNCs resembled human neurons early in development, expressed a variety of neuroprogenitor and neuronal genes as well as several neuroprogenitor and neuronal proteins and also presented electrical activity. In addition, when these neuronal-like cells were exposed to either dopamine or colchicine, they responded similarly to neurons by retracting their neuronal arborizations. More importantly, MDNCs exhibited reproducible differentiation rates, arborizations and expression of dopamine 1 receptors (DR1) on separate sequential samples from the same individual. Differentiation efficiency measured by cell morphology was on average 11.9 ± 1.4% (mean, SEM, n = 38,819 cells from 15 donors). To provide context and help researchers decide which in vitro model of neuronal development is best suited to address their scientific question,we compared our results with those of other in vitro models currently available and exposed advantages and disadvantages of each paradigm.</p>