10.3389/fphar.2018.01547.s001 Luiz Henrique César Vasconcelos Luiz Henrique César Vasconcelos Maria da Conceição Correia Silva Maria da Conceição Correia Silva Alana Cristina Costa Alana Cristina Costa Giuliana Amanda de Oliveira Giuliana Amanda de Oliveira Iara Leão Luna de Souza Iara Leão Luna de Souza Fernando Ramos Queiroga Fernando Ramos Queiroga Layanne da Cunha Araujo Layanne da Cunha Araujo Glêbia Alexa Cardoso Glêbia Alexa Cardoso Renato Fraga Righetti Renato Fraga Righetti Alexandre Sérgio Silva Alexandre Sérgio Silva Patrícia Mirella da Silva Patrícia Mirella da Silva Carla Roberta de Oliveira Carvalho Carla Roberta de Oliveira Carvalho Giciane Carvalho Vieira Giciane Carvalho Vieira Iolanda de Fátima Lopes Calvo Tibério Iolanda de Fátima Lopes Calvo Tibério Fabiana de Andrade Cavalcante Fabiana de Andrade Cavalcante Bagnólia Araújo da Silva Bagnólia Araújo da Silva Data_Sheet_1_A Guinea Pig Model of Airway Smooth Muscle Hyperreactivity Induced by Chronic Allergic Lung Inflammation: Contribution of Epithelium and Oxidative Stress.pdf Frontiers 2019 airways asthma contractile reactivity relaxation oxidative stress 2019-01-24 04:16:46 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_A_Guinea_Pig_Model_of_Airway_Smooth_Muscle_Hyperreactivity_Induced_by_Chronic_Allergic_Lung_Inflammation_Contribution_of_Epithelium_and_Oxidative_Stress_pdf/7622384 <p>Asthma is a heterogeneous disease of the airways characterized by chronic inflammation associated with bronchial and smooth muscle hyperresponsiveness. Currently, different murine models for the study of asthma show poor bronchial hyperresponsiveness due to a scarcity of smooth muscle and large airways, resulting in a failure to reproduce smooth muscle hyperreactivity. Thus, we aimed to standardize a guinea pig model of chronic allergic lung inflammation mimicking airway smooth muscle hyperreactivity observed in asthmatics (Asth). Animals were randomly divided into a control group (Ctrl), which received saline (0.9% NaCl), and the Asth group, subjected to in vivo sensitization with ovalbumin (OVA) nebulization. Morphological analysis was performed by hematoxylin-eosin staining. Bronchial hyperresponsiveness was evaluated by nebulization time in the fifth, sixth, and seventh inhalations (NT5-7) and tracheal isometric contractions were assessed by force transducer. Total antioxidant capacity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and protein expression by Western blot. Histologically, the Asth group developed peribronchial cellular infiltrate, epithelial hyperplasia and smooth muscle thickening. After the fourth nebulization, the Asth group developed bronchial hyperreactivity. The trachea from the Asth group contracted after in vitro stimulation with OVA, differing from the Ctrl group, which showed no response. Additionally, airway smooth muscle hyperreactivity to carbachol and histamine was observed in the Asth group only in intact epithelium preparations, but not to KCl, and this effect was associated with an augmented production of reactive oxygen species. Moreover, lung inflammation impaired the relaxant potency of isoproterenol only in intact epithelium preparations, without interfering with nifedipine, and it was found to be produced by transforming growth factor-β negative modulation of β adrenergic receptors and, furthermore, big-conductance Ca<sup>2+</sup>-sensitive K<sup>+</sup> channels. These effects were also associated with increased levels of phosphatidylinositol 3-kinases but not extracellular signal-regulated kinases 1/2 or phosphorylation, and augmented α-actin content as well, explaining the increased smooth muscle mass. Furthermore, pulmonary antioxidant capacity was impaired in the Asth group. Therefore, we developed a standardized and easy-to-use, reproducible guinea pig model of lung inflammation that mimics airway smooth muscle hypercontractility, facilitating the investigation of the mechanisms of bronchial hyperresponsiveness in asthma and new therapeutic alternatives.</p>