10.3389/fpls.2018.01979.s003
Qibin Ma
Qibin
Ma
Zhenglin Xia
Zhenglin
Xia
Zhandong Cai
Zhandong
Cai
Lu Li
Lu
Li
Yanbo Cheng
Yanbo
Cheng
Jia Liu
Jia
Liu
Hai Nian
Hai
Nian
Table_1_GmWRKY16 Enhances Drought and Salt Tolerance Through an ABA-Mediated Pathway in Arabidopsis thaliana.DOCX
Frontiers
2019
GmWRKY16
soybean
salt
drought
ABA
Arabidopsis thaliana
2019-01-21 04:17:39
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_GmWRKY16_Enhances_Drought_and_Salt_Tolerance_Through_an_ABA-Mediated_Pathway_in_Arabidopsis_thaliana_DOCX/7610378
<p>The WRKY transcription factors (TFs) are one of the largest families of TFs in plants and play multiple roles in plant development and stress response. In the present study, GmWRKY16 encoding a WRKY transcription factor in soybean was functionally characterized in Arabidopsis. GmWRKY16 is a nuclear protein that contains a highly conserved WRKY domain and a C<sub>2</sub>H<sub>2</sub> zinc-finger structure, and has the characteristics of transcriptional activation ability, presenting a constitutive expression pattern with relative expression levels of over fourfold in the old leaves, flowers, seeds and roots of soybean. The results of quantitative real time polymerase chain reaction (qRT-PCR) showed that GmWRKY16 could be induced by salt, alkali, ABA, drought and PEG-6000. As compared with the control, overexpression of GmWRKY16 in Arabidopsis increased the seed germination rate and root growth of seedlings in transgenic lines under higher concentrations of mannitol, NaCl and ABA. In the meantime, GmWRKY16 transgenic lines showed over 75% survival rate after rehydration and enhanced Arabidopsis tolerance to salt and drought with higher proline and lower MDA accumulation, less water loss of the detached leaves, and accumulated more endogenous ABA than the control under stress conditions. Further studies showed that AtWRKY8, KIN1, and RD29A were induced in GmWRKY16 transgenic plants under NaCl treatment. The expressions of the ABA biosynthesis gene (NCED3), signaling genes (ABI1, ABI2, ABI4, and ABI5), responsive genes (RD29A, COR15A, COR15B, and RD22) and stress-related marker genes (KIN1, LEA14, LEA76, and CER3) were regulated in transgenic lines under drought stress. In summary, these results suggest that GmWRKY16 as a WRKY TF may promote tolerance to drought and salt stresses through an ABA-mediated pathway.</p>