10.3389/fimmu.2018.02990.s004
Ben Wylie
Ben
Wylie
James Read
James
Read
Anthony C. Buzzai
Anthony C.
Buzzai
Teagan Wagner
Teagan
Wagner
Niamh Troy
Niamh
Troy
Genevieve Syn
Genevieve
Syn
Shane R. Stone
Shane
R. Stone
Bree Foley
Bree
Foley
Anthony Bosco
Anthony
Bosco
Mark N. Cruickshank
Mark N.
Cruickshank
Jason Waithman
Jason
Waithman
Image_3_CD8+XCR1neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors.TIFF
Frontiers
2019
dendritic cells
TLR5
XCR1
immunosurveillance
transcriptomics
microarray
2019-01-16 14:46:18
Figure
https://frontiersin.figshare.com/articles/figure/Image_3_CD8_XCR1neg_Dendritic_Cells_Express_High_Levels_of_Toll-Like_Receptor_5_and_a_Unique_Complement_of_Endocytic_Receptors_TIFF/7594454
<p>Conventional dendritic cells (cDC) resident in the lymphoid organs of mice have been classically divided into CD8<sup>+</sup> and CD8<sup>neg</sup> subsets. It is well-established that CD8<sup>+</sup> dendritic cells (DCs) and their migratory counterparts in the periphery comprise the cross-presenting cDC1 subset. In contrast, CD8<sup>neg</sup> DCs are grouped together in the heterogeneous cDC2 subset. CD8<sup>neg</sup> DCs are relatively poor cross-presenters and drive more prominent CD4<sup>+</sup> T cell responses against exogenous antigens. The discovery of the X-C motif chemokine receptor 1 (XCR1) as a specific marker of cross-presenting DCs, has led to the identification of a divergent subset of CD8<sup>+</sup> DCs that lacks the ability to cross-present. Here, we report that these poorly characterized CD8<sup>+</sup>XCR1<sup>neg</sup> DCs have a gene expression profile that is consistent with both plasmacytoid DCs (pDCs) and cDC2. Our data demonstrate that CD8<sup>+</sup>XCR1<sup>neg</sup> DCs possess a unique pattern of endocytic receptors and a restricted toll-like receptor (TLR) profile that is particularly enriched for TLR5, giving them a unique position within the DC immunosurveillance network.</p>