10.3389/fimmu.2018.02990.s004 Ben Wylie Ben Wylie James Read James Read Anthony C. Buzzai Anthony C. Buzzai Teagan Wagner Teagan Wagner Niamh Troy Niamh Troy Genevieve Syn Genevieve Syn Shane R. Stone Shane R. Stone Bree Foley Bree Foley Anthony Bosco Anthony Bosco Mark N. Cruickshank Mark N. Cruickshank Jason Waithman Jason Waithman Image_3_CD8+XCR1neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors.TIFF Frontiers 2019 dendritic cells TLR5 XCR1 immunosurveillance transcriptomics microarray 2019-01-16 14:46:18 Figure https://frontiersin.figshare.com/articles/figure/Image_3_CD8_XCR1neg_Dendritic_Cells_Express_High_Levels_of_Toll-Like_Receptor_5_and_a_Unique_Complement_of_Endocytic_Receptors_TIFF/7594454 <p>Conventional dendritic cells (cDC) resident in the lymphoid organs of mice have been classically divided into CD8<sup>+</sup> and CD8<sup>neg</sup> subsets. It is well-established that CD8<sup>+</sup> dendritic cells (DCs) and their migratory counterparts in the periphery comprise the cross-presenting cDC1 subset. In contrast, CD8<sup>neg</sup> DCs are grouped together in the heterogeneous cDC2 subset. CD8<sup>neg</sup> DCs are relatively poor cross-presenters and drive more prominent CD4<sup>+</sup> T cell responses against exogenous antigens. The discovery of the X-C motif chemokine receptor 1 (XCR1) as a specific marker of cross-presenting DCs, has led to the identification of a divergent subset of CD8<sup>+</sup> DCs that lacks the ability to cross-present. Here, we report that these poorly characterized CD8<sup>+</sup>XCR1<sup>neg</sup> DCs have a gene expression profile that is consistent with both plasmacytoid DCs (pDCs) and cDC2. Our data demonstrate that CD8<sup>+</sup>XCR1<sup>neg</sup> DCs possess a unique pattern of endocytic receptors and a restricted toll-like receptor (TLR) profile that is particularly enriched for TLR5, giving them a unique position within the DC immunosurveillance network.</p>