10.3389/fonc.2018.00659.s009 Xiang-Yu Meng Xiang-Yu Meng Ming-Jun Shi Ming-Jun Shi Zi-Hang Zeng Zi-Hang Zeng Chen Chen Chen Chen Tong-Zu Liu Tong-Zu Liu Qiu-Ji Wu Qiu-Ji Wu Shuo Li Shuo Li Sheng Li Sheng Li Table_8_The Role of COL5A2 in Patients With Muscle-Invasive Bladder Cancer: A Bioinformatics Analysis of Public Datasets Involving 787 Subjects and 29 Cell Lines.xlsx Frontiers 2019 muscle-invasive bladder cancer COL5A2 prognosis IPD meta-analysis bioinformatics 2019-01-15 04:04:28 Dataset https://frontiersin.figshare.com/articles/dataset/Table_8_The_Role_of_COL5A2_in_Patients_With_Muscle-Invasive_Bladder_Cancer_A_Bioinformatics_Analysis_of_Public_Datasets_Involving_787_Subjects_and_29_Cell_Lines_xlsx/7586720 <p>Bladder cancer (BC) is one of the most common malignancies. Two previous studies identified collagen type V alpha 2 (COL5A2) as a potential biomarker in BC, both are simple reanalysis of a single transcriptomic dataset without subgroup analysis for muscle-invasive BC (MIBC). We focused in MIBC patients and explored the role of COL5A2 from an integration perspective, using refined methodology covering individual participant data meta-analysis and bioinformatics analysis. Eight transcriptomic datasets of 787 MIBC patients (including one dataset containing genomic mutation information) and two drug sensitivity datasets of 29 cell lines in which more than 250 compounds were analyzed. We found subjects with increased COL5A2 gene expression exhibited poorer prognosis, and the power analysis confirmed adequate sample size. FGFR3 was the only gene differential mutated between the COL5A2 high and low expression groups. Differential expression and co-expression network analysis suggested potential association between COL5A2 expression and essential pathways involved in cancer invasion and dissemination, including cell adhesion, extracellular matrix organization, and epithelial-mesenchymal transition. Coordinately, analysis of drug screening datasets and gene-drug interaction also revealed COL5A2 expression linked to cell morphogenesis, angiogenesis, blood vessel development, and urogenital development. The utility and feasibility of COL5A2 for clinically applicable prognosis prediction and risk classification and the exact underlying molecular mechanism should be further investigated in subsequent studies.</p>