10.3389/fimmu.2018.03099.s001
Qiongyi Hu
Qiongyi
Hu
Wen Gong
Wen
Gong
Jieyu Gu
Jieyu
Gu
Guannan Geng
Guannan
Geng
Ting Li
Ting
Li
Rui Tian
Rui
Tian
Zhitao Yang
Zhitao
Yang
Haocheng Zhang
Haocheng
Zhang
Lingyun Shao
Lingyun
Shao
Tingting Liu
Tingting
Liu
Liyan Wan
Liyan
Wan
Jinchao Jia
Jinchao
Jia
Chengde Yang
Chengde
Yang
Yi Shi
Yi
Shi
Hui Shi
Hui
Shi
Data_Sheet_1_Plasma microRNA Profiles as a Potential Biomarker in Differentiating Adult-Onset Still's Disease From Sepsis.pdf
Frontiers
2019
adult-onset still's disease
AOSD
sepsis
microRNAs
biomarker
inflammation
2019-01-11 13:32:21
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Plasma_microRNA_Profiles_as_a_Potential_Biomarker_in_Differentiating_Adult-Onset_Still_s_Disease_From_Sepsis_pdf/7577888
<p>Adult-onset Still's disease (AOSD) is a systemic inflammatory disease characterized by cytokine storm. However, a diagnostic test for AOSD in clinical use is yet to be validated. The aim of our study was to identify non-invasive biomarkers with high specificity and sensitivity to diagnosis of AOSD. MicroRNA (miRNA) profiles in PBMC from new-onset AOSD patients without any treatment and healthy controls (HCs) were analyzed by miRNA deep sequencing. Plasma samples from 100 AOSD patients and 60 HCs were used to validated the expression levels of miRNA by qRT-PCR. The correlations between expression levels of miRNAs and clinical manifestations were analyzed using advanced statistical models. We found that plasma samples from AOSD patients showed a distinct miRNA expression profile. Five miRNAs (miR-142-5p, miR-101-3p, miR-29a-3p, miR-29c-3p, and miR-141-3p) were significantly upregulated in plasma of AOSD patients compared with HCs both in training and validation sets. We discovered a panel including 3 miRNAs (miR-142-5p, miR-101-3p, and miR-29a-3p) that can predict the probability of AOSD with an area under the receiver operating characteristic (ROC) curve of 0.8250 in training and validation sets. Moreover, the expression levels of 5 miRNAs were significantly higher in active AOSD patients compared with those in inactive patients. In addition, elevated level of miR-101-3p was found in AOSD patients with fever, sore throat and arthralgia symptoms; the miR-101-3p was also positively correlated with the levels of IL-6 and TNF-α in serum. Furthermore, five miRNAs (miR-142-5p, miR-101-3p, miR-29c-3p, miR-29a-3p, and miR-141-3p) expressed in plasma were significantly higher in AOSD patients than in sepsis patients (P < 0.05). The AUC value of 4-miRNA panel (miR-142-5p, miR-101-3p, miR-29c-3p, and miR-141-3p) for AOSD diagnosis from sepsis was 0.8448, revealing the potentially diagnostic value to distinguish AOSD patients from sepsis patients. Our results have identified a specific plasma miRNA signature that may serve as a potential non-invasive biomarker for diagnosis of AOSD and monitoring disease activity.</p>