10.3389/fimmu.2018.02550.s002 Rajeev K. Tyagi Rajeev K. Tyagi Nikunj Tandel Nikunj Tandel Richa Deshpande Richa Deshpande Robert W. Engelman Robert W. Engelman Satish D. Patel Satish D. Patel Priyanka Tyagi Priyanka Tyagi Image_1_Humanized Mice Are Instrumental to the Study of Plasmodium falciparum Infection.tif Frontiers 2018 humanized/chimeric mice malaria NSG mice TK/NOG mice FRG mice huRBCs huHep clodronate loaded liposomes 2018-12-13 04:07:26 Figure https://frontiersin.figshare.com/articles/figure/Image_1_Humanized_Mice_Are_Instrumental_to_the_Study_of_Plasmodium_falciparum_Infection_tif/7459451 <p>Research using humanized mice has advanced our knowledge and understanding of human haematopoiesis, non-adaptive and adaptive immunity, autoimmunity, infectious disease, cancer biology, and regenerative medicine. Challenges posed by the human-malaria parasite Plasmodium falciparum include its complex life cycle, the evolution of drug resistance against anti-malarials, poor diagnosis, and a lack of effective vaccines. Advancements in genetically engineered and immunodeficient mouse strains, have allowed for studies of the asexual blood stage, exoerythrocytic stage and the transition from liver-to-blood stage infection, in a single vertebrate host. This review discusses the process of “humanization” of various immunodeficient/transgenic strains and their contribution to translational biomedical research. Our work reviews the strategies employed to overcome the remaining-limitations of the developed human-mouse chimera(s).</p>