10.3389/fmicb.2018.01075.s002 Xixi Cai Xixi Cai Lingyan Huang Lingyan Huang Guiqin Yang Guiqin Yang Zhen Yu Zhen Yu Junlin Wen Junlin Wen Shungui Zhou Shungui Zhou Table_3_Transcriptomic, Proteomic, and Bioelectrochemical Characterization of an Exoelectrogen Geobacter soli Grown With Different Electron Acceptors.pdf Frontiers 2018 comparative transcriptomics comparative proteomics bioelectrochemistry Geobacter soli extracellular electron transfer 2018-12-12 08:16:26 Dataset https://frontiersin.figshare.com/articles/dataset/Table_3_Transcriptomic_Proteomic_and_Bioelectrochemical_Characterization_of_an_Exoelectrogen_Geobacter_soli_Grown_With_Different_Electron_Acceptors_pdf/7454216 <p>The ability of Geobacter species to transfer electrons outside cells enables them to play an important role in biogeochemical and bioenergy processes. Our knowledge of the extracellular electron transfer (EET) process in the genus Geobacter is mainly from the study of G. sulfurreducens, and in order to fully investigate the EET mechanisms in the genus Geobacter, other Geobacter species should also be considered. This study focused on the EET of Geobacter soli GSS01, which exhibited a capability of reducing insoluble Fe(III) oxides and generating electrical current comparable with G. sulfurreducens PCA. Electrochemical characterization, including cyclic voltammetry, differential pulse voltammetry, and electrochemical in situ FTIR spectra, revealed that different redox proteins contributed to the electrochemical behaviors of G. soli and G. sulfurreducens. Based on comparative transcriptomic and proteomic analyses, OmcS was the most upregulated protein in both G. soli and G. sulfurreducens cells grown with insoluble Fe(III) oxides vs. soluble electron acceptor. However, the proteins including OmcE and PilA that were previously reported as being important for EET in G. sulfurreducens were downregulated or unchanged in G. soli cells grown with insoluble electron acceptors vs. soluble electron acceptor, and many proteins that were upregulated in G. soli cells grown with insoluble electron acceptors vs. soluble electron acceptor, such as OmcN, are not important for EET in G. sulfurreducens. We also identified 30 differentially expressed small RNAs (sRNAs) in G. soli cells grown with different acceptors. Taken together, these findings help to understand the versatile EET mechanisms that exist in the genus Geobacter and point to the possibility of sRNA in modulating EET gene expression.</p>