10.3389/fimmu.2018.02844.s001 Rui Zhang Rui Zhang Lin Xu Lin Xu Dong Zhang Dong Zhang Bo Hu Bo Hu Qi Luo Qi Luo Dan Han Dan Han Jiangbing Li Jiangbing Li Chengwu Shen Chengwu Shen Data_Sheet_1_Cardioprotection of Ginkgolide B on Myocardial Ischemia/Reperfusion-Induced Inflammatory Injury via Regulation of A20-NF-κB Pathway.docx Frontiers 2018 Ginkgolide B Myocardial ischemia/reperfusion injury Inflammation A20 NF-κB 2018-12-12 04:18:35 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Cardioprotection_of_Ginkgolide_B_on_Myocardial_Ischemia_Reperfusion-Induced_Inflammatory_Injury_via_Regulation_of_A20-NF-_B_Pathway_docx/7453352 <p>Inflammation urges most of the characteristics of plaques involved in the pathogenesis of myocardial ischemia/reperfusion injury (MI/RI). In addition, inflammatory signaling pathways not only mediate the properties of plaques that precipitate ischemia/reperfusion (I/R) but also influence the clinical consequences of the post-infarction remodeling and heart failure. Here, we studied whether Ginkgolide B (GB), an effective anti-inflammatory monomer, improved MI/RI via suppression of inflammation. Left anterior descending (LAD) coronary artery induced ischemia/reperfusion (I/R) of rats or A20 silencing mice, as well as hypoxia/reoxygenation (H/R) induced damages of primary cultured rat neonatal ventricular myocytes or A20 silencing ventricular myocytes, respectively, served as MI/RI model in vivo and in vitro to discuss the anti-I/R injury properties of GB. We found that GB significantly alleviated the symptoms of MI/RI evidently by reducing infarct size, preventing ultrastructural changes of myocardium, depressing Polymorphonuclears (PMNs) infiltration, lessening histopathological damage and suppressing the excessive inflammation. Further study demonstrated that GB remarkably inhibited NF-κB p65 subunit translocation, IκB-α phosphorylation, IKK-β activity, as well as the downstream inflammatory cytokines and proteins expressions via zinc finger protein A20. In conclusion, GB could alleviate MI/RI-induced inflammatory response through A20-NF-κB signal pathway, which may give us new insights into the preventive strategies for MI/RI disease.</p>