10.3389/fimmu.2018.02813.s003 Markus Bardua Markus Bardua Claudia Haftmann Claudia Haftmann Pawel Durek Pawel Durek Kerstin Westendorf Kerstin Westendorf Antje Buttgereit Antje Buttgereit Cam Loan Tran Cam Loan Tran Mairi McGrath Mairi McGrath Melanie Weber Melanie Weber Katrin Lehmann Katrin Lehmann Richard Kwasi Addo Richard Kwasi Addo Gitta Anne Heinz Gitta Anne Heinz Anna-Barbara Stittrich Anna-Barbara Stittrich Patrick Maschmeyer Patrick Maschmeyer Helena Radbruch Helena Radbruch Michael Lohoff Michael Lohoff Hyun-Dong Chang Hyun-Dong Chang Andreas Radbruch Andreas Radbruch Mir-Farzin Mashreghi Mir-Farzin Mashreghi Table_2_MicroRNA-31 Reduces the Motility of Proinflammatory T Helper 1 Lymphocytes.xlsx Frontiers 2018 CD4 miR-31 miRNA target identification T cell migration Th1 cells regulatory networks antagomirs 2018-12-06 04:03:14 Dataset https://frontiersin.figshare.com/articles/dataset/Table_2_MicroRNA-31_Reduces_the_Motility_of_Proinflammatory_T_Helper_1_Lymphocytes_xlsx/7428113 <p>Proinflammatory type 1 T helper (Th1) cells are enriched in inflamed tissues and contribute to the maintenance of chronic inflammation in rheumatic diseases. Here we show that the microRNA- (miR-) 31 is upregulated in murine Th1 cells with a history of repeated reactivation and in memory Th cells isolated from the synovial fluid of patients with rheumatic joint disease. Knock-down of miR-31 resulted in the upregulation of genes associated with cytoskeletal rearrangement and motility and induced the expression of target genes involved in T cell activation, chemokine receptor– and integrin-signaling. Accordingly, inhibition of miR-31 resulted in increased migratory activity of repeatedly activated Th1 cells. The transcription factors T-bet and FOXO1 act as positive and negative regulators of T cell receptor (TCR)–mediated miR-31 expression, respectively. Taken together, our data show that a gene regulatory network involving miR-31, T-bet, and FOXO1 controls the migratory behavior of proinflammatory Th1 cells.</p>