10.3389/fmicb.2018.02787.s012
Qi Liu
Qi
Liu
Horst Kämpf
Horst
Kämpf
Robert Bussert
Robert
Bussert
Patryk Krauze
Patryk
Krauze
Fabian Horn
Fabian
Horn
Tobias Nickschick
Tobias
Nickschick
Birgit Plessen
Birgit
Plessen
Dirk Wagner
Dirk
Wagner
Mashal Alawi
Mashal
Alawi
Data_Sheet_8_Influence of CO2 Degassing on the Microbial Community in a Dry Mofette Field in Hartoušov, Czech Republic (Western Eger Rift).CSV
Frontiers
2018
geo–bio interaction
elevated CO2 concentration
paleo-sediment
deep biosphere
acidophilic microorganisms
Acidobacteriaceae
Acidithiobacillus
Acidothermus
2018-11-21 04:12:05
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_8_Influence_of_CO2_Degassing_on_the_Microbial_Community_in_a_Dry_Mofette_Field_in_Hartou_ov_Czech_Republic_Western_Eger_Rift_CSV/7368650
<p>The Cheb Basin (CZ) is a shallow Neogene intracontinental basin filled with fluvial and lacustrine sediments that is located in the western part of the Eger Rift. The basin is situated in a seismically active area and is characterized by diffuse degassing of mantle-derived CO<sub>2</sub> in mofette fields. The Hartoušov mofette field shows a daily CO<sub>2</sub> flux of 23–97 tons of CO<sub>2</sub> released over an area of 0.35 km<sup>2</sup> and a soil gas concentration of up to 100% CO<sub>2</sub>. The present study aims to explore the geo–bio interactions provoked by the influence of elevated CO<sub>2</sub> concentrations on the geochemistry and microbial community of soils and sediments. To sample the strata, two 3-m cores were recovered. One core stems from the center of the degassing structure, whereas the other core was taken 8 m from the ENE and served as an undisturbed reference site. The sites were compared regarding their geochemical features, microbial abundances, and microbial community structures. The mofette site is characterized by a low pH and high TOC/sulfate contents. Striking differences in the microbial community highlight the substantial impact of elevated CO<sub>2</sub> concentrations and their associated side effects on microbial processes. The abundance of microbes did not show a typical decrease with depth, indicating that the uprising CO<sub>2</sub>-rich fluid provides sufficient substrate for chemolithoautotrophic anaerobic microorganisms. Illumina MiSeq sequencing of the 16S rRNA genes and multivariate statistics reveals that the pH strongly influences microbial composition and explains around 38.7% of the variance at the mofette site and 22.4% of the variance between the mofette site and the undisturbed reference site. Accordingly, acidophilic microorganisms (e.g., OTUs assigned to Acidobacteriaceae and Acidithiobacillus) displayed a much higher relative abundance at the mofette site than at the reference site. The microbial community at the mofette site is characterized by a high relative abundance of methanogens and taxa involved in sulfur cycling. The present study provides intriguing insights into microbial life and geo–bio interactions in an active seismic region dominated by emanating mantle-derived CO<sub>2</sub>-rich fluids, and thereby builds the basis for further studies, e.g., focusing on the functional repertoire of the communities. However, it remains open if the observed patterns can be generalized for different time-points or sites.</p>