10.3389/fimmu.2018.02655.s007
Patricia Castro-Sánchez
Patricia
Castro-Sánchez
Rocío Ramirez-Munoz
Rocío
Ramirez-Munoz
Noa B. Martín-Cófreces
Noa
B. Martín-Cófreces
Oscar Aguilar-Sopeña
Oscar
Aguilar-Sopeña
Sergio Alegre-Gomez
Sergio
Alegre-Gomez
Sara Hernández-Pérez
Sara
Hernández-Pérez
Raquel Reyes
Raquel
Reyes
Qi Zeng
Qi
Zeng
Carlos Cabañas
Carlos
Cabañas
Francisco Sánchez-Madrid
Francisco
Sánchez-Madrid
Pedro Roda-Navarro
Pedro
Roda-Navarro
Video_6_Phosphatase of Regenerating Liver-1 (PRL-1) Regulates Actin Dynamics During Immunological Synapse Assembly and T Cell Effector Function.AVI
Frontiers
2018
T cell immune response
immunological synapse
phosphatases of regenerating liver
actin cytoskeleton
IL-2
2018-11-20 04:08:57
Media
https://frontiersin.figshare.com/articles/media/Video_6_Phosphatase_of_Regenerating_Liver-1_PRL-1_Regulates_Actin_Dynamics_During_Immunological_Synapse_Assembly_and_T_Cell_Effector_Function_AVI/7361675
<p>The regulatory role of most dual specific phosphatases during T cell activation remains unknown. Here, we have studied the expression and function of phosphatases of regenerating liver (PRLs: PRL-1, PRL-2, and PRL-3) during T cell activation, as well as, the dynamic delivery of PRL-1 to the Immunological Synapse (IS). We found that T cell activation downregulates the expression of PRL-2, resulting in an increased PRL-1/PRL-2 ratio. PRL-1 redistributed at the IS in two stages: Initially, it was transiently accumulated at scanning membranes enriched in CD3 and actin, and at later times, it was delivered at the contact site from pericentriolar, CD3ζ-containing, vesicles. Once at the established IS, PRL-1 distributed to LFA-1 and CD3ε sites. Remarkably, PRL-1 was found to regulate actin dynamics during IS assembly and the secretion of IL-2. Moreover, pharmacological inhibition of the catalytic activity of the three PRLs reduced the secretion of IL-2. These results provide evidence indicating a regulatory role of PRL-1 during IS assembly and highlight the involvement of PRLs in immune responses by mature T cells.</p>