I. Santamaría, Ramón Sevillano, Laura Martín, Jesús Genilloud, Olga González, Ignacio Díaz, Margarita Data_Sheet_8_The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces.PDF <p>The xenobiotic response element (XRE) transcription factors belong to a regulator family frequently found in Streptomyces that are often followed by small proteins with a DUF397 domain. In fact, the pair XRE-DUF397 has been proposed to comprise toxin–antitoxin (TA) type II systems. In this work, we demonstrate that one of these putative TA-systems, encoded by the genes SCO4441 and SCO4442 of Streptomyces coelicolor, and denominated Scr1/Scr2 (which stands for S. coelicolorregulator), does not behave as a toxin–antitoxin system under the conditions used as was originally expected. Instead the pair Scr1/Scr2 acts as a strong positive regulator of endogenous antibiotic production in S. coelicolor. The analysis of the 19 Streptomyces strains tested determined that overexpression of the pair Scr1/Scr2 drastically induces the production of antibiotics not only in S. coelicolor, but also in Streptomyces lividans, Streptomyces peucetius, Streptomyces steffisburgensis and Streptomyces sp. CA-240608. Our work also shows that Scr1 needs Scr2 to exert positive regulation on antibiotic production.</p> Streptomyces;positive regulator;antibiotic production;xenobiotic response element;toxin–antitoxin 2018-11-16
    https://frontiersin.figshare.com/articles/dataset/Data_Sheet_8_The_XRE-DUF397_Protein_Pair_Scr1_and_Scr2_Acts_as_a_Strong_Positive_Regulator_of_Antibiotic_Production_in_Streptomyces_PDF/7350779
10.3389/fmicb.2018.02791.s008