%0 Generic %A I. Santamaría, Ramón %A Sevillano, Laura %A Martín, Jesús %A Genilloud, Olga %A González, Ignacio %A Díaz, Margarita %D 2018 %T Data_Sheet_6_The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces.PDF %U https://frontiersin.figshare.com/articles/dataset/Data_Sheet_6_The_XRE-DUF397_Protein_Pair_Scr1_and_Scr2_Acts_as_a_Strong_Positive_Regulator_of_Antibiotic_Production_in_Streptomyces_PDF/7350773 %R 10.3389/fmicb.2018.02791.s006 %2 https://frontiersin.figshare.com/ndownloader/files/13582772 %K Streptomyces %K positive regulator %K antibiotic production %K xenobiotic response element %K toxin–antitoxin %X

The xenobiotic response element (XRE) transcription factors belong to a regulator family frequently found in Streptomyces that are often followed by small proteins with a DUF397 domain. In fact, the pair XRE-DUF397 has been proposed to comprise toxin–antitoxin (TA) type II systems. In this work, we demonstrate that one of these putative TA-systems, encoded by the genes SCO4441 and SCO4442 of Streptomyces coelicolor, and denominated Scr1/Scr2 (which stands for S. coelicolorregulator), does not behave as a toxin–antitoxin system under the conditions used as was originally expected. Instead the pair Scr1/Scr2 acts as a strong positive regulator of endogenous antibiotic production in S. coelicolor. The analysis of the 19 Streptomyces strains tested determined that overexpression of the pair Scr1/Scr2 drastically induces the production of antibiotics not only in S. coelicolor, but also in Streptomyces lividans, Streptomyces peucetius, Streptomyces steffisburgensis and Streptomyces sp. CA-240608. Our work also shows that Scr1 needs Scr2 to exert positive regulation on antibiotic production.

%I Frontiers