10.3389/fphys.2018.01608.s005
Weixing Zhang
Weixing
Zhang
Wenfeng Chen
Wenfeng
Chen
Zhenfang Li
Zhenfang
Li
Lanting Ma
Lanting
Ma
Jing Yu
Jing
Yu
Hongfang Wang
Hongfang
Wang
Zhenguo Liu
Zhenguo
Liu
Baohua Xu
Baohua
Xu
Image_5_Identification and Characterization of Three New Cytochrome P450 Genes and the Use of RNA Interference to Evaluate Their Roles in Antioxidant Defense in Apis cerana cerana Fabricius.TIF
Frontiers
2018
Apis cerana cerana Fabricius
cytochrome P450s
RNA interference
abiotic stresses
antioxidant enzyme
2018-11-15 14:36:00
Figure
https://frontiersin.figshare.com/articles/figure/Image_5_Identification_and_Characterization_of_Three_New_Cytochrome_P450_Genes_and_the_Use_of_RNA_Interference_to_Evaluate_Their_Roles_in_Antioxidant_Defense_in_Apis_cerana_cerana_Fabricius_TIF/7346834
<p>Cytochrome P450s play critical roles in maintaining redox homeostasis and protecting organisms from the accumulation of toxic reactive oxygen species (ROS). The biochemical functions of the P450 family have essentially been associated with the metabolism of xenobiotics. Here, we sequenced and characterized three P450 genes, AccCYP314A1, AccCYP4AZ1, and AccCYP6AS5, from Apis cerana cerana Fabricius; these genes play a critical role in maintaining biodiversity. Quantitative PCR (qPCR) analysis indicated that the three genes were all predominantly expressed in the epidermis (EP), followed by the brain (BR) and midgut (MG). In addition, the highest expression levels were detected in the dark-eyed pupae and adult stages. The three genes were induced by temperature (4°C and 44°C), heavy metals (CdCl<sub>2</sub> and HgCl<sub>2</sub>), pesticides (DDV, deltamethrin, and paraquat) and UV treatments. Furthermore, Western blot analysis indicated that the protein expression levels could be induced by some abiotic stressors, a result that complements the qPCR results. We analyzed the silencing of these three genes and found that silencing these genes enhanced the enzymatic activities of peroxidase (POD) and catalase (CAT). Additionally, we investigated the expression of other antioxidant genes and found that some were upregulated, while others were downregulated, suggesting that the upregulated genes may be involved in compensating for the silencing of AccCYP314A1, AccCYP4AZ1, and AccCYP6AS5. Our findings suggest that AccCYP314A1, AccCYP4AZ1, and AccCYP6AS5 may play very significant roles in the antioxidant defense against damage caused by ROS.</p>