10.3389/fcimb.2018.00369.s011
Hugo Costa Paes
Hugo Costa
Paes
Lorena da Silveira Derengowski
Lorena
da Silveira Derengowski
Luisa Defranco Ferreira Peconick
Luisa Defranco Ferreira
Peconick
Patrícia Albuquerque
Patrícia
Albuquerque
Georgios Joannis Pappas
Georgios Joannis
Pappas
André Moraes Nicola
André Moraes
Nicola
Fabiana Brandão Alves Silva
Fabiana Brandão Alves
Silva
Marcelo Afonso Vallim
Marcelo Afonso
Vallim
J. Andrew Alspaugh
J.
Andrew Alspaugh
Maria Sueli Soares Felipe
Maria
Sueli Soares Felipe
Larissa Fernandes
Larissa
Fernandes
Video_2_A Wor1-Like Transcription Factor Is Essential for Virulence of Cryptococcus neoformans.MP4
Frontiers
2018
Cryptococcus neoformans
capsule
cytokinesis
virulence
transcription factor
2018-11-13 11:10:44
Media
https://frontiersin.figshare.com/articles/media/Video_2_A_Wor1-Like_Transcription_Factor_Is_Essential_for_Virulence_of_Cryptococcus_neoformans_MP4/7333907
<p>Gti1/Pac2 transcription factors occur exclusively in fungi and their roles vary according to species, including regulating morphological transition and virulence, mating and secondary metabolism. Many of these functions are important for fungal pathogenesis. We therefore hypothesized that one of the two proteins of this family in Cryptococcus neoformans, a major pathogen of humans, would also control virulence-associated cellular processes. Elimination of this protein in C. neoformans results in reduced polysaccharide capsule expression and defective cytokinesis and growth at 37°C. The mutant loses virulence in a mouse model of cryptococcal infection and retains only partial virulence in the Galleria mellonella alternative model at 30°C. We performed RNA-Seq experiments on the mutant and found abolished transcription of genes that, in combination, are known to account for all the observed phenotypes. The protein has been named Required for cytokinesis and virulence 1 (Rcv1).</p>