10.3389/fcimb.2018.00369.s011 Hugo Costa Paes Hugo Costa Paes Lorena da Silveira Derengowski Lorena da Silveira Derengowski Luisa Defranco Ferreira Peconick Luisa Defranco Ferreira Peconick Patrícia Albuquerque Patrícia Albuquerque Georgios Joannis Pappas Georgios Joannis Pappas André Moraes Nicola André Moraes Nicola Fabiana Brandão Alves Silva Fabiana Brandão Alves Silva Marcelo Afonso Vallim Marcelo Afonso Vallim J. Andrew Alspaugh J. Andrew Alspaugh Maria Sueli Soares Felipe Maria Sueli Soares Felipe Larissa Fernandes Larissa Fernandes Video_2_A Wor1-Like Transcription Factor Is Essential for Virulence of Cryptococcus neoformans.MP4 Frontiers 2018 Cryptococcus neoformans capsule cytokinesis virulence transcription factor 2018-11-13 11:10:44 Media https://frontiersin.figshare.com/articles/media/Video_2_A_Wor1-Like_Transcription_Factor_Is_Essential_for_Virulence_of_Cryptococcus_neoformans_MP4/7333907 <p>Gti1/Pac2 transcription factors occur exclusively in fungi and their roles vary according to species, including regulating morphological transition and virulence, mating and secondary metabolism. Many of these functions are important for fungal pathogenesis. We therefore hypothesized that one of the two proteins of this family in Cryptococcus neoformans, a major pathogen of humans, would also control virulence-associated cellular processes. Elimination of this protein in C. neoformans results in reduced polysaccharide capsule expression and defective cytokinesis and growth at 37°C. The mutant loses virulence in a mouse model of cryptococcal infection and retains only partial virulence in the Galleria mellonella alternative model at 30°C. We performed RNA-Seq experiments on the mutant and found abolished transcription of genes that, in combination, are known to account for all the observed phenotypes. The protein has been named Required for cytokinesis and virulence 1 (Rcv1).</p>