%0 Generic %A Diaz, Julia M. %A Holland, Alisia %A Sanders, James G. %A Bulski, Karrie %A Mollett, Douglas %A Chou, Chau-Wen %A Phillips, Dennis %A Tang, Yuanzhi %A Duhamel, Solange %D 2018 %T Table_1_Dissolved Organic Phosphorus Utilization by Phytoplankton Reveals Preferential Degradation of Polyphosphates Over Phosphomonoesters.xlsx %U https://frontiersin.figshare.com/articles/dataset/Table_1_Dissolved_Organic_Phosphorus_Utilization_by_Phytoplankton_Reveals_Preferential_Degradation_of_Polyphosphates_Over_Phosphomonoesters_xlsx/7251776 %R 10.3389/fmars.2018.00380.s001 %2 https://frontiersin.figshare.com/ndownloader/files/13356254 %K dissolved organic phosphorus %K alkaline phosphatase activity %K polyphosphate %K phosphoester %K phosphoanhydride %K phosphorus stress %K Thalassiosira %K diatom %X

The nutritionally available pool of dissolved organic phosphorus (DOP) supports marine primary productivity in a range of ocean ecosystems but remains poorly resolved. Here, the relative lability of model phosphorus (P) compounds representing the major P(V) bond classes of marine DOP – phosphomonoesters (P-O-C) and phosphoanhydrides (P-O-P) – was assessed in diatom cultures of the genus Thalassiosira, as well as coastal field sites of the western North Atlantic. In diatom samples, maximum enzymatic hydrolysis rates revealed that the P-anhydride bonds of inorganic tripolyphosphate (3poly-P), followed by the P-anhydride bonds of adenosine 5′-triphosphate (ATP), were preferentially degraded relative to the P-monoesters adenosine 5′-monophosphate (AMP) and 4-methylumbelliferone phosphate (MUF-P). Consistent with these rate measurements, targeted proteomics analysis demonstrated that the underlying phosphatase diversity present in diatom samples was dominated by P-anhydride degrading enzymes (inorganic pyrophosphatases and nucleoside triphosphatases). Furthermore, biomass-normalized rates of ATP degradation were always suppressed under P-replete conditions in diatom cultures, but the effect of overall P availability on 3poly-P degradation was inconsistent among diatom strains, suggesting that inorganic polyphosphate (poly-P) degradation may persist irrespective of prevailing P levels in the marine environment. Indeed, the majority of field sites examined in the P-replete coastal western North Atlantic exhibited significantly higher maximum rates of inorganic poly-P hydrolysis relative to P-monoester hydrolysis, which was largely driven by phytoplankton dynamics. Based on these results, the possibility that P-anhydride utilization may contribute comparably or even more substantially than P-esters to community-level P demand, phytoplankton growth, and primary productivity should be considered.

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