Jia, Honglei Chen, Sisi Liu, Dan Liesche, Johannes Shi, Cong Wang, Juan Ren, Meijuan Wang, Xiaofeng Yang, Jun Shi, Wei Li, Jisheng Table_1_Ethylene-Induced Hydrogen Sulfide Negatively Regulates Ethylene Biosynthesis by Persulfidation of ACO in Tomato Under Osmotic Stress.DOC <p>A number of recent studies identified hydrogen sulfide (H<sub>2</sub>S) as an important signal in plant development and adaptation to environmental stress. H<sub>2</sub>S has been proven to participate in ethylene-induced stomatal closure, but how the signaling pathways of H<sub>2</sub>S and ethylene interact is still unclear. Here, we reveal how H<sub>2</sub>S controls the feedback-regulation of ethylene biosynthesis in tomato (Solanum lycopersicum) under osmotic stress. We found that ethylene induced the production of H<sub>2</sub>S in guard cells. The supply of hypotaurine (HT; a H<sub>2</sub>S scavenger) or DL-pro-pargylglycine (PAG; a synthetic inhibitor of H<sub>2</sub>S) removed the effect of ethylene or osmotic stress on stomatal closure. This suggests that ethylene-induced H<sub>2</sub>S is a downstream component of osmotic stress signaling, which is required for ethylene-induced stomatal closure under osmotic stress. We further found that H<sub>2</sub>S inhibited ethylene synthesis through inhibiting the activity of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidases (ACOs) by persulfidation. A modified biotin-switch method (MBST) showed that H<sub>2</sub>S can induce persulfidation of LeACO1 and LeACO2 in a dose-dependent manner, and that persulfidation inhibits the activity of LeACO1 and LeACO2. We also found that LeACO1 is persulfidated at cysteine 60. These data suggested that ethylene-induced H<sub>2</sub>S negatively regulates ethylene biosynthesis by persulfidation of LeACOs. In addition, H<sub>2</sub>S was also found to inhibit the expression of LeACO genes. The results provide insight on the general mode of action of H<sub>2</sub>S and contribute to a better understanding of a plant’s response to osmotic stress.</p> hydrogen sulfide;ethylene;persulfidation;feedback regulation;tomato;LeACOs;abiotic stress 2018-10-17
    https://frontiersin.figshare.com/articles/dataset/Table_1_Ethylene-Induced_Hydrogen_Sulfide_Negatively_Regulates_Ethylene_Biosynthesis_by_Persulfidation_of_ACO_in_Tomato_Under_Osmotic_Stress_DOC/7217999
10.3389/fpls.2018.01517.s001