10.3389/fimmu.2018.02355.s002
Stefania De Santis
Stefania
De Santis
Maria R. Fiorentino
Maria R.
Fiorentino
Vanessa Galleggiante
Vanessa
Galleggiante
Giulio Verna
Giulio
Verna
Marina Liso
Marina
Liso
Monica Massaro
Monica
Massaro
Jinggang Lan
Jinggang
Lan
Jacopo Troisi
Jacopo
Troisi
Alessia Bertamino
Alessia
Bertamino
Aldo Pinto
Aldo
Pinto
Pietro Campiglia
Pietro
Campiglia
Angelo Santino
Angelo
Santino
Gianluigi Giannelli
Gianluigi
Giannelli
Alessio Fasano
Alessio
Fasano
Grazia Serino
Grazia
Serino
Marcello Chieppa
Marcello
Chieppa
Image_2_Aquaporin 9 Contributes to the Maturation Process and Inflammatory Cytokine Secretion of Murine Dendritic Cells.TIF
Frontiers
2018
AQP9
dendritic cells
inflammation
il-12
glycerol
2018-10-16 04:44:37
Figure
https://frontiersin.figshare.com/articles/figure/Image_2_Aquaporin_9_Contributes_to_the_Maturation_Process_and_Inflammatory_Cytokine_Secretion_of_Murine_Dendritic_Cells_TIF/7211003
<p>Dendritic cells (DCs) are the most potent antigen-presenting cells able to trigger the adaptive immune response to specific antigens. When non-self-antigens are captured, DCs switch from an “immature” to a “mature” state to fulfill their function. Among the several surface proteins involved in DCs maturation, the role of aquaporins (AQPs) is still poorly understood. Here we investigated the expression profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significantly upregulated 6 h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. Despite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected from DSS induced colitis. All together, our data indicate that AQP9 blockade can be an efficient strategy to reduce DCs inflammatory response but it is not sufficient to protect from acute inflammatory insults such as DSS induced colitis.</p>